Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
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DOI：
10.1016/s1097-2765(00)80296-8
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发表时间：
1998-12-01
期刊：
影响因子：
16
通讯作者：
Cortes, P
中科院分区：
文献类型：
--
作者：
Besmer, E;Mansilla-Soto, J;Cortes, P
尽管发夹打开在抗原受体基因组装中很重要，但介导这一反应的分子机制尚未确定。在这里，我们证明了RAG1和RAG2可以打开DNA发夹。RAG的发夹打开不是序列特异性的，但在镁离子中，发夹打开只发生在受调控的切割复合体的背景下。RAGS打开发夹的化学机制类似于HIV整合酶和Mu转座酶对RSS的裂解和3‘端的加工，这些反应可以通过醇解进行，干扰RSS裂解的RAGI或RAG2突变也会干扰发夹打开，这表明RAG有一个催化几个不同DNA裂解反应的单一活性部位。
Despite the importance of hairpin opening in antigen receptor gene assembly, the molecular machinery that mediates this reaction has not been defined. Here, we show that RAG1 plus RAG2 can open DNA hairpins. Hairpin opening by RAGs is not sequence specific, but in Mg2+, hairpin opening occurs only in the context of a regulated cleavage complex. The chemical mechanism of hairpin opening by RAGs resembles RSS cleavage and 3' end processing by HIV integrase and Mu transposase in that these reactions can proceed through alcoholysis, Mutations in either RAGI or RAG2 that interfere with RSS cleavage also interfere with hairpin opening, suggesting that RAGs have a single active site that catalyzes several distinct DNA cleavage reactions.