Food deprivation promotes oxidative imbalance in rat brain.
Food deprivation promotes oxidative imbalance in rat brain.
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DOI：
10.1111/j.1750-3841.2008.00982.x
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发表时间：
2009
影响因子：
3.9
通讯作者：
Renato X Santos;S. Cardoso;Sónia Silva;S. Correia;C. Carvalho;J. Crisóstomo;L. Rodrigues;C. Amaral;T. Louro;P. Matafome;Maria S. Santos;T. Proença;Ana I. Duarte;R. Seiça;Paula I. Moreira
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文献类型：
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作者：
Renato X Santos;S. Cardoso;Sónia Silva;S. Correia;C. Carvalho;J. Crisóstomo;L. Rodrigues;C. Amaral;T. Louro;P. Matafome;Maria S. Santos;T. Proença;Ana I. Duarte;R. Seiça;Paula I. Moreira
本研究旨在评价食物剥夺对Wistar和Goto-Kakizaki（GK）大鼠脑氧化状态的影响。为此，我们评估了几个氧化应激参数：脂质过氧化（硫代巴比妥酸反应物质[TBARS]）和蛋白质氧化标志物，过氧化氢（H（2）O（2））水平，非酶（还原型[GSH]和氧化型谷胱甘肽[GSSG]和维生素E）和酶（谷胱甘肽过氧化物酶[GPx]，谷胱甘肽还原酶[GRed]和锰超氧化物歧化酶[MnSOD]）抗氧化防御。4月龄Wistar和GK大鼠分为2组。在2个月期间，每种大鼠品系的一组维持在正常饮食下，其他组维持在50%食物剥夺下。正常饮食的GK大鼠维生素E水平较低，而GRd活性和GSH/GSSG比值较高。在Wistar大鼠中，在促氧化剂对ADP/Fe（2+）存在的情况下，食物剥夺诱导维生素E水平显著降低，GPx活性、H2 O产生和TBARS形成显著增加。然而，与正常饮食的GK大鼠相比，食物剥夺下的GK大鼠的维生素E水平和GRd活性显著降低，而H（2）O（2）产生显著增加。总之，我们的研究结果表明，食物剥夺会影响大脑的氧化状态，这可能使脑细胞易于退化和死亡。
The present study was aimed to evaluate the effect of food deprivation in brain oxidative status of Wistar and Goto-Kakizaki (GK) rats. For this purpose, we evaluated several oxidative stress parameters: lipid peroxidation (thiobarbituric acid reactive substances [TBARS]) and protein oxidation markers, hydrogen peroxide (H(2)O(2)) levels, nonenzymatic (reduced [GSH] and oxidized glutathione [GSSG] and vitamin E) and enzymatic (glutathione peroxidase [GPx], glutathione reductase [GRed], and manganese superoxide dismutase [MnSOD]) antioxidant defenses. Four-mo-old Wistar and GK rats were divided into 2 groups. One group of each rat strain was maintained under normal diet and the other groups were maintained under 50% food deprivation during 2 mo. GK rats under normal diet presented lower levels of vitamin E and higher GRed activity and GSH/GSSG ratio when compared with Wistar control rats. In Wistar rats, food deprivation induced a significant decrease in vitamin E levels and a significant increase in GPx activity, H(2)O(2) production, and TBARS formation in the presence of the prooxidant pair ADP/Fe(2+). However, GK rats under food deprivation presented a significant decrease in vitamin E levels and GRed activity and a significant increase in H(2)O(2) production when compared with GK under normal diet. In summary, our results indicate that food deprivation affects brain oxidative status, which could predispose brain cells to degeneration and death.