Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI medaka (transgenic Oryzias latipes) exposed to ethinylestradiol
Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI medaka (transgenic Oryzias latipes) exposed to ethinylestradiol
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DOI：
10.1021/es0620134
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发表时间：
2007-02-15
影响因子：
11.4
通讯作者：
Honjo, Tsuneo
中科院分区：
文献类型：
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作者：
Hano, Takeshi;Oshima, Yuji;Honjo, Tsuneo
本研究探讨了乙烯基雌二醇(EE2)对转基因水稻OVAS-GFP/ST II-Yi Medaka成熟性腺的不良和持续性影响。测量性腺大小，计算GFP荧光面积，是一种能够用GFP荧光监测活体标本中性腺的技术。首先，将成熟青竹暴露于EE2(47.8-522 ng/L)中4周。染毒剂量为216 ng/L的雄鼠和522 ng/L的雌鼠的性腺GFP荧光面积和性腺指数均显著降低。组织学观察显示，所有处理组的雄鼠均出现睾丸卵子，此外，当剂量为216 ng/L时，结缔组织发生率较高。接下来，将成熟雄鼠暴露于剂量为43.7-473 ng/L的EE2 3周并净化6周，以研究EE2的持续影响。性腺连续观察发现，~gt；=215 ng/L初始暴露3周后，GFP开始下降，净化后性腺荧光区逐渐恢复，至净化期末无统计学差异，精子发生正常。绿色荧光蛋白荧光的变化清楚地表明了转基因青竹的性腺状况，该菌株显示了一种便于筛选的鱼类模型，以检测雌激素化学物质对性腺的不利影响。
This study investigates the adverse and persistent effects of ethinylestradiol (EE2) on mature gonads of transgenic olvas-GFP/ST II-YI medaka (Oryzias latipes). The measurement of gonadal size calculating the GFP-fluorescent area was used as a technique that enabled monitoring gonads in living specimens by GFP fluorescence. First, mature medaka were exposed to EE2 (47.8-522 ng/L) for 4 weeks. The gonads showed a significant reduction of the GFP-fluorescent area and Gonadosomatic Index in males exposed to EE2 at > 216 ng/L and females exposed at 522 ng/L. Histologically, males at all treatments exhibited testis-ova and additionally, high connective tissue prevalence at >= 216 ng/L. Next, mature male medaka were exposed to EE2 (43.7-473 ng/L) for 3 weeks and allowed to depurate for 6 weeks, to investigate persistent effects of EE2. Continuous gonad observation showed that GFP began to decline 3 weeks after initial exposure to >= 215 ng/L. After depuration, the gonad's fluorescent areas gradually recovered, with no statistical difference at the end of the depuration period; normal spermatogenesis was present in these individuals. Alterations in GFP fluorescence clearly indicate the condition of the gonad in transgenic medaka and this strain showed a facilitated screening fish model to detect the adverse effects on the gonad by estrogenic chemicals.