Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk
Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk
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DOI：
10.1016/j.biortech.2012.08.136
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发表时间：
2012-12-01
影响因子：
11.4
通讯作者：
Mori, Yutaka
中科院分区：
文献类型：
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作者：
Prawitwong, Panida;Kosugi, Akihiko;Mori, Yutaka
为了有效利用淀粉和纤维素两种物质，油棕榈树干被分为薄壁组织(PA)和维管束(VB)。高固相同时糖化发酵(HSS-SSF)采用30%(w/v)PA，含46.7%(w/w)淀粉，辅以淀粉酶和酿酒酵母K3，可生产6.1%(w/v)乙醇。然后用无淀粉PA(SFPA)和VB进行氢氧化钠碱预处理。在每克底物中添加18FPU的商业纤维素酶和10U的Novozyme-188时，5%(w/v)的SFPA和VB的酶消化率分别为92%和97%。同样，使用30%(w/v)碱处理的SFPA和VB、纤维素酶和酵母，HSS-SSF的乙醇产量分别为8.2%和8.5%(w/v)。这些结果表明，分离PA和VB的HSS-SSF是一种在不损失淀粉和纤维物质的情况下适用于油棕榈树干的发酵策略。(C)2012爱思唯尔有限公司。保留所有权利。
For efficient utilization of both starchy and cellulosic materials, oil palm trunk was separated into parenchyma (PA) and vascular bundle (VB). High solid-state simultaneous saccharification and fermentation (HSS-SSF) using 30% (w/v) PA, containing 46.7% (w/w) starch, supplemented with amylases and Saccharomyces cerevisiae K3, produced 6.1% (w/v) ethanol. Subsequent alkali-pretreatment using sodium hydroxide was carried out with starch-free PA (sfPA) and VB. Enzymatic digestibility of 5% (w/v) pretreated sfPA and VB was 92% and 97%, respectively, using 18 FPU of commercial cellulase supplemented with 10 U of Novozyme-188 per gram of substrate. Likewise, HSS-SSF using 30% (w/v) alkali-pretreated sfPA and VB, with cellulases and yeast, resulted in high ethanol production (8.2% and 8.5% (w/v), respectively). These results show that HSS-SSF using separated PA and VB is a useful fermentation strategy, without loss of starchy and cellulosic materials, for oil palm trunk. (c) 2012 Elsevier Ltd. All rights reserved.