MORPHOLOGICAL PATHWAY OF FLAGELLAR ASSEMBLY IN SALMONELLA-TYPHIMURIUM
MORPHOLOGICAL PATHWAY OF FLAGELLAR ASSEMBLY IN SALMONELLA-TYPHIMURIUM
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DOI：
10.1016/0022-2836(92)90958-m
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发表时间：
1992-07-20
影响因子：
5.6
通讯作者：
AIZAWA, SI
中科院分区：
文献类型：
--
作者：
KUBORI, T;SHIMAMOTO, N;AIZAWA, SI
研究了鼠伤寒沙门氏菌鞭毛的组装过程。用氯化铯密度梯度法对不同鞭毛突变体产生的7种鞭毛前体进行纯化。它们的特征在于形态学的电子显微镜，和生化的二维凝胶电泳。MS环是在没有任何其他鞭毛成分的情况下形成的，包括开关复合物和推定的输出装置。先前鉴定为杆状组分的四种蛋白质FlgB、FlgC、FlgF、FlgG和另一种蛋白质FliE协同组装成稳定的结构。钩在两个不同的步骤中形成;其近端部分的形成和伸长。在没有LP环的情况下，近端部分形成，但未发生伸长。FlgD是钩形成所必需的，但不是LP环形成所必需的。根据这些结果和其他结果，提出了一个修订的鞭毛组装途径。
The process of flagellar assembly was investigated inSalmonella typhimurium. Seven types of flagellar precursors produced by various flagellar mutants were purified by CsCl density gradient protocol. They were characterized morphologically by electron microscopy, and biochemically by two-dimensional gel electrophoresis. The MS ring is formed in the absence of any other flagellar components, including the switch complex and the putative export apparatus. Four proteins previously identified as rod components, FlgB, FlgC, FlgF, FlgG, and another protein, FliE, assemble co-operatively into a stable structure. The hook is formed in two distinct steps; formation of its proximal part and elongation. Proximal part formation occurs, but elongation does not occur, in the absence of the LP ring. FlgD is necessary for hook formation, but not for LP-ring formation. A revised pathway of flagellar assembly is proposed based on these and other results.