Evaluation of the inflammatory response in sera from acute ischemic stroke patients by measurement of IL-2 and IL-10
Evaluation of the inflammatory response in sera from acute ischemic stroke patients by measurement of IL-2 and IL-10
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DOI：
10.1007/s00011-009-0036-4
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发表时间：
2009-10-01
影响因子：
6.7
通讯作者：
Daginawala, Hatim F.
中科院分区：
文献类型：
--
作者：
Nayak, Amit R.;Kashyap, Rajpal S.;Daginawala, Hatim F.
我们比较了急性缺血性脑卒中（AIS）患者血清中促炎细胞因子白细胞介素-2 （IL-2）和抗炎细胞因子白细胞介素-10 （IL-10）的浓度，以确定其预后价值。在入院时以及入院后24、48、72和144小时采集症状出现后24小时内入院的AIS患者（n = 17）的血液。采用酶联免疫吸附法测定促炎性和抗炎性细胞因子。AIS患者血清IL-2水平在0 （0.97,P < 0.01）和24 h （1.011, P < 0.01）时升高。改善患者（n = 13）的IL-2浓度在随后的时间点逐渐下降（48、72和144 h， 0.324, P < 0.05），而死亡患者（n = 4）的IL-2浓度则没有下降（n = 4）。同样，AIS患者血清样本中IL-10水平下降，在24 h时改善（0.180,P < 0.05），随后在72 h （0.97, P < 0.01）和144 h （1.38, P < 0.01）显著升高。IL-2和IL-10的水平与AIS患者的预后密切相关，可能对预后有帮助。
We compared the concentrations of the proinflammatory cytokine interleukin-2 (IL-2) with the anti-inflammatory cytokine interleukin-10 (IL-10) in serial serum samples from improved and expired acute ischemic stroke (AIS) patients to determine their prognostic usefulness.Blood from AIS patients (n = 17) admitted within 24 h of the onset of symptoms were collected at admission and 24, 48, 72, and 144 h after admission. Pro- and anti-inflammatory cytokines were measured by enzyme-linked immunosorbant assay.IL-2 levels were elevated in serum samples from AIS patients collected at 0 (0.97, P < 0.01) and 24 h (1.011, P < 0.01). IL-2 concentrations gradually decreased at subsequent time-points (48, 72, and 144 h samples, 0.324, P < 0.05) in patients who improved (n = 13), but not in those who expired (n = 4). Similarly, a decrease in IL-10 levels was observed in serum samples from AIS patients who improved at 24 h (0.180, P < 0.05), followed by significant increases at 72 h (0.97, P < 0.01 vs. control) and 144 h (1.38, P < 0.01).The levels of IL-2 and IL-10 correlate well with outcome in AIS patients and may be useful in prognosis.