Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein
Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein
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DOI：
10.1021/bi036155b
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发表时间：
2004-05-25
期刊：
影响因子：
2.9
通讯作者：
Olejniczak, ET
中科院分区：
文献类型：
--
作者：
Huang, QL;Yu, LP;Olejniczak, ET
严重急性呼吸综合征（SARS）病毒属于冠状病毒科病毒。它的病毒颗粒编码多种蛋白质，包括复制酶和四种结构蛋白。在这里，我们描述了 SARS 冠状病毒 (CoV) 核衣壳蛋白 N 末端结构域的三维结构。该蛋白由五链 β 片层组成，其折叠拓扑结构不同于其他 RNA 结合蛋白。单链 RNA 在柔性、带正电荷的 β 发夹和核心结构之间的连接处结合到蛋白质表面。基于核磁共振的筛选用于识别与该位点结合的低分子量化合物。
The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.