Sensitive determination of the phosphodiesterase III/IV inhibitor zardaverine in human serum by direct sample injection, automated precolumn clean-up and high-performance liquid chromatography.
Sensitive determination of the phosphodiesterase III/IV inhibitor zardaverine in human serum by direct sample injection, automated precolumn clean-up and high-performance liquid chromatography.
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DOI：
10.1016/s0021-9673(01)88473-9
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发表时间：
1991-08
期刊：
影响因子：
--
通讯作者：
R. Huber;U. Schildnitz
中科院分区：
文献类型：
--
作者：
R. Huber;U. Schildnitz
描述了一种高效液相色谱法测定磷酸二酯酶III/IV抑制剂扎达维林在血清中使用全自动清洗大体积的血清样品上的半制备规模的预柱，然后通过色谱上的两个分析柱操作与两种不同的溶剂系统。分析柱的切换提供了必要的专属性，并且通过样品体积获得了UV检测的足够灵敏度。该方法显示出几乎定量的回收率，允许使用外标定量。在1-50 ng/ml浓度范围内，可证明具有良好的精密度和线性。该方法适用于常规测量，以支持人体中扎达维林的动力学研究。
A high-performance liquid chromatographic method is described for the determination of the phosphodiesterase III/IV inhibitor zardaverine in serum by using fully automated clean-up of large-volume serum samples on a semi-preparative-scale precolumn followed by chromatography on two analytical columns operated with two different solvent systems. The switching of the analytical columns provides the necessary specificity and sufficient sensitivity for UV detection is obtained by the sample volume. The method was shown to give nearly quantitative recovery, allowing the use of external standard quantification. Good precision and linearity within the concentration range 1-50 ng/ml could be demonstrated. The method is suitable for routine measurements in support of kinetic studies of zardaverine in man.