Acclimation of metabolism to light in Arabidopsis thaliana: the glucose 6-phosphate/phosphate translocator GPT2 directs metabolic acclimation.
Acclimation of metabolism to light in Arabidopsis thaliana: the glucose 6-phosphate/phosphate translocator GPT2 directs metabolic acclimation.
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DOI：
10.1111/pce.12495
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Johnson GN
中科院分区：
文献类型：
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作者：
Dyson BC;Allwood JW;Feil R;Xu Y;Miller M;Bowsher CG;Goodacre R;Lunn JE;Johnson GN
植物的成熟叶片从低光转移到强光通常会增加它们的光合能力。在拟南芥中，这种动态适应需要GPT 2的表达，GPT 2是一种葡萄糖6-磷酸/磷酸转运蛋白。在这里，我们研究了GPT 2对叶片代谢和光合作用的影响。野生型和GPT 2基因敲除（gpt2.2）的植物在低光下生长，尽管具有不同的代谢和转录组策略，但获得了相同的光合速率。在转移到强光下后，gpt 2.2植物立即显示出比野生型植物更高的光合速率（35%）;然而，在随后的几天里，野生型植物适应了光合能力，在7天后光合速率增加了100%。在整个驯化过程中，野生型植物比gpt 2.2植物积累更多的淀粉。我们认为GPT 2活性导致6-磷酸葡萄糖从细胞质净输入叶绿体，增加淀粉合成。有明显的代谢适应，短期变化通常被逆转为植物适应。在野生型和gpt 2.2叶中观察到对光的不同响应。在gpt 2.2中观察到显著更高水平的糖磷酸盐。我们认为GPT 2改变了代谢物在隔室之间的分布，这在细胞解释环境信号方面起着至关重要的作用。适应环境的变化对优化植物生长和生产力至关重要。在拟南芥中，葡萄糖-6-磷酸/磷酸转运蛋白GPT 2在适应增加的辐照中起着重要作用。在这里，我们表明，GPT 2的表达影响不同的存储池之间的碳分配，增强淀粉合成。代谢的驯化发生，但在表达或缺乏GPT 2 γ的植物中是不同的。
Mature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In A rabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photosynthesis. Plants of wild type and of a GPT2 knockout (gpt2.2) grown under low light achieved the same photosynthetic rate despite having different metabolic and transcriptomic strategies. Immediately upon transfer to high light, gpt2.2 plants showed a higher rate of photosynthesis than wild‐type plants (35%); however, over subsequent days, wild‐type plants acclimated photosynthetic capacity, increasing the photosynthesis rate by 100% after 7 d. Wild‐type plants accumulated more starch than gpt2.2 plants throughout acclimation. We suggest that GPT2 activity results in the net import of glucose 6‐phosphate from cytosol to chloroplast, increasing starch synthesis. There was clear acclimation of metabolism, with short‐term changes typically being reversed as plants acclimated. Distinct responses to light were observed in wild‐type and gpt2.2 leaves. Significantly higher levels of sugar phosphates were observed in gpt2.2. We suggest that GPT2 alters the distribution of metabolites between compartments and that this plays an essential role in allowing the cell to interpret environmental signals. Acclimation to changes in the environment are crucial to optimising plant growth and productivity. In Arabidopsis thaliana, the glucose‐6‐phosphate/phosphate translocator GPT2 plays an important role in the acclimation to increased irradiance. Here we show that expression of GPT2 influences the partitioning of carbon between different storage pools, enhancing starch synthesis. Acclimation of metabolism occurs but is distinct in plants expressing or lacking GPT2.y