Cloning and expression of a plastid-encoded subunit, beta-carboxyltransferase gene (accD) and a nuclear-encoded subunit, biotin carboxylase of acetyl-CoA carboxylase from oil palm (Elaeis guineensis Jacq.)
Cloning and expression of a plastid-encoded subunit, beta-carboxyltransferase gene (accD) and a nuclear-encoded subunit, biotin carboxylase of acetyl-CoA carboxylase from oil palm (Elaeis guineensis Jacq.)
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DOI：
10.1016/j.plantsci.2008.05.023
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发表时间：
2008-10-01
期刊：
影响因子：
5.2
通讯作者：
Phongdara, Amornrat
中科院分区：
文献类型：
--
作者：
Nakkaew, Alisa;Chotigeat, Witaiwan;Phongdara, Amornrat
棕榈油是世界市场上第二大贸易油脂，棕榈是主要为其油而种植的最重要的作物。鉴定了油棕的品种，具有高含油量并通过选择性育种程序生产的棕榈油，对于提高油的产量，随后提高在包括生物柴油的各种应用中使用油棕的经济可行性是理想的。我们从大肠杆菌中克隆了生物素羧化酶（accD）基因。几内亚花该基因编码异聚乙酰辅酶A羧化酶（ACCase）的质体编码亚基。accD基因的cDNA（登录号DQ 004687）具有一个1479 bp的开放阅读框，编码492个氨基酸残基的蛋白质（AAY 86362），预测分子量为55.47 kDa。ACC酶的异聚体形式是重要的，因为它催化脂肪酸合成的第一个关键步骤。有证据共同表明，质体中accD的表达对异聚体ACC酶的水平至关重要，进而对植物中种子油的量至关重要。在这里，我们支持的假设，accD的表达水平与油棕产量通过使用半定量逆转录聚合酶链反应（RT-PCR）和定量实时聚合酶链反应（实时PCR）分析。此外，我们观察到类似的表达谱的核编码亚基，生物素羧化酶（accC）。这一发现代表了与植物高产相关的表达基因的遗传背景，并且ACCase可以用作育种程序中的标记。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Palm oil is the second largest traded oil or fat in the world market and palm is the most important crop grown mainly for its oil. Identified varieties of Elaeis guineensis jacq., with a high oil content and produced through a selective breeding program, are desirable for improving the yield of oil, subsequently enhancing the economic feasibility of using oil palm in various applications, including bio-diesel. We have cloned the gene of biotin carboxylase (accD) from E. guineensis Jacq. This gene encodes a plastid-coded subunit of heteromeric acetyl-CoA carboxylase (ACCase). The cDNA of accD gene (accession number DQ004687) has an open reading frame of 1479 bp that encodes a putative protein of 492 amino acid residues (AAY86362) with a predicted molecular mass of 55.47 kDa. The heteromeric form of ACCase is important as it catalyzes the first committed step of fatty acid synthesis. There is evidence that collectively suggests that the expression of accD in plastids is crucial to the levels of heteromeric ACCase and in turn, to the amount of seed oil in plant. Here we support the hypothesis that the expression level of accD is correlated with the oil palm production by using a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time polymerase chain reaction (real-time PCR) analysis. Moreover, we observed the similar expression profile in nuclear-encoded subunit, biotin carboxylase (accC). This finding represents the genetic background of the expressed genes that correlate to high yield in plant and that ACCase can be used as a marker in the breeding program. (c) 2008 Elsevier Ireland Ltd. All rights reserved.