Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells.
Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells.
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微流控循环肿瘤细胞的分离和单细胞分析。
DOI：
10.1007/978-1-0716-3323-6_7
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Fan,ZHugh
中科院分区：
文献类型：
--
作者：
Le,Minh-ChauN;Smith,KierstinA;Alipanah,Morteza;Chen,Kangfu;Lagmay,JoanneP;Fan,ZHugh
微流控平台可以浓缩和分析循环肿瘤细胞(CTCs)，这是一种潜在的癌症诊断、预后和治疗的生物标志物。结合免疫细胞化学/免疫荧光(ICC/IF)检测CTCs，微流控检测为研究肿瘤异质性和预测治疗反应提供了独特的机会，这两者都有助于癌症药物的开发。在本章中，我们详细介绍了用于从肉瘤患者的血液样本中浓缩、检测和分析单个CTCs的微流控装置的制作和方法。
Microfluidic platforms enable the enrichment and analysis of circulating tumor cells (CTCs), a potential biomarker for cancer diagnosis, prognosis, and theragnosis. Combined with immunocytochemistry/immunofluorescence (ICC/IF) assays for CTCs, microfluidics-enabled detection presents a unique opportunity to study tumor heterogeneity and predict treatment response, both of which can help cancer drug development. In this chapter, we detail the protocols and methods employed to fabricate and use a microfluidic device for the enrichment, detection, and analysis of single CTCs from the blood samples of sarcoma patients.