Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings
Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings
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DOI：
10.3109/10425179809008472
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Hasegawa, K
中科院分区：
文献类型：
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作者：
Anai, T;Kono, N;Hasegawa, K
从萝卜的cDNA和基因组文库中分离到一个与大豆生长素诱导的SAUR（Small Auxin UpRNAs）基因同源的基因。该cDNA克隆长约470 bp，在S ′-非翻译区含有一个与mRNA不稳定性有关的DST（Down STlymphocyte）元件。该基因组克隆在其启动子区含有两个与生长素相关的核心序列（AGTCTC和TATCCCAC）。萝卜SAUR转录本在生长素和放线菌酮处理中以与其他植物相同的方式积累。然而，在所有其他SAUR基因启动子中报道的NDE和DUE元件在该萝卜SAUR基因中不保守。结果表明，SAUR基因的启动子活性将仅由两个短序列调节。
A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the S'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promoters were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.