The potential role of ribosomal protein s5 on cell cycle arrest and initiation of murine erythroleukernia cell differentiation
The potential role of ribosomal protein s5 on cell cycle arrest and initiation of murine erythroleukernia cell differentiation
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DOI：
10.1002/jcb.21722
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发表时间：
2008-07-01
影响因子：
4
通讯作者：
Vizirianakis, Ioannis S.
中科院分区：
文献类型：
--
作者：
Matragkou, Christina N.;Papachristou, Eleni T.;Vizirianakis, Ioannis S.
现在有证据表明，一些核糖体蛋白除了是核糖体亚基的结构成分外，还参与了细胞分化和凋亡的调节。如前所述，小鼠红白血病(MEL)细胞红系分化的启动与编码核糖体RNAs和核糖体蛋白S5(RPS5)和L35a的基因转录失活有关。在这项研究中，我们扩展了这些观察，并研究了转RPS5基因的MEL细胞是否影响了红系成熟起始和细胞周期停滞的开始。建立稳定转染的MEL克隆细胞(MEL-C14和MEL-C56)，并对其产生RPS5 RNA转录本及其翻译产物的能力进行评价。RPS5基因对Mel细胞RPS5基因表达模式和RPS5蛋白积聚的影响与其启动分化，(B)进入G(1)/G(0)期细胞周期停滞，以及(C)调控细胞周期蛋白依赖性激酶CDK2、CDK4和CDK6的水平有关。这些数据表明，RPS5基因表达(结构性表达)的解除影响了RPS5的蛋白水平，并推迟了诱导剂处理的MEL细胞启动红系成熟和进入细胞周期停滞的时间。
Evidence now exists to indicate that some ribosomal proteins besides being structural components of the ribosomal subunits are involved in the regulation of cell differentiation and apoptosis. As we have shown earlier, initiation of erythroid differentiation of murine erythroleukemia (MEL) cel Is is associated with transcriptional inactivation of genes encoding ribosomal RNAs and ribosomal proteins S5 (RPS5) and L35a. In this study, we extended these observations and investigated whether transfection of MEL cells with RPS5cDNA affects the onset of initiation of erythroid maturation and their entrance in cell cycle arrest. Stably transfected MEL cloned cells (MEL-C14 and MEL-C56) were established and assessed for their capacity to produce RPS5 RNA transcript and its translated product. The impact of RPS5 cDNA transfection on the RPS5gene expression patterns and the accumulation of RPS5 protein in inducible transfected MEL cel Is werecorrelated with theirabilityto: (a) initiate differentiation, (b) enter cell cycle arrest at G(1)/G(0) phase, and (c) modulate the level of cyclin-dependent kinases CDK2, CDK4, and CDK6. The data presented indicate that deregulation of RPS5 gene expression (constitutive expression) affects RPS5 protein level and delays both the onset of initiation of erythroid maturation and entrance in cell cycle arrest in inducer-treated MEL cells.