RNAi-mediated Ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions
RNAi-mediated Ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions
复制标题
DOI：
10.1111/pbi.12013
复制
发表时间：
2013-04-01
影响因子：
13.8
通讯作者：
Rathore, Keerti S.
中科院分区：
文献类型：
--
作者：
Palle, Sreenath R.;Campbell, LeAnne M.;Rathore, Keerti S.
棉籽仍然是皮棉生产的低价值副产品，主要是由于存在有毒棉酚，使其不适合单胃动物。利用RNAi技术敲低陆地棉β-杜松烯合酶基因，获得了超低酚棉籽（ULGCS）品系。本研究的目的是评估ULGCS性状的稳定性和特异性，并评估转基因株系的农艺性能。经过30年的试验表明，ULGCS性状在田间条件下是稳定的，转基因品系的叶/花器官含有野生型水平的棉酚和相关萜类化合物。虽然这是一个相对小规模的研究，我们没有观察到任何负面影响，无论是产量或质量的纤维和种子的转基因株系相比，非转基因亲本植物。对2009年期间从田间生长的植物获得的种子进行成分分析。正如预期的那样，ULGCS和野生型棉籽之间的主要差异在于它们的棉酚水平。除含油量外，ULGCS的组成与非转基因棉籽相似。有趣的是，ULGCS与来自非转基因亲本的种子相比具有显著更高的油含量（4%~ 8%）。田间试验结果证实了ULGCS性状的稳定性和特异性，表明这种基于RNAi的产品具有商业可行性的潜力。因此，有可能提高和扩大年度棉籽产量的营养效用，以满足人类日益增长的需求。
Cottonseed remains a low-value by-product of lint production mainly due to the presence of toxic gossypol that makes it unfit for monogastrics. Ultra-low gossypol cottonseed (ULGCS) lines were developed using RNAi knockdown of -cadinene synthase gene(s) in Gossypium hirsutum. The purpose of the current study was to assess the stability and specificity of the ULGCS trait and evaluate the agronomic performance of the transgenic lines. Trials conducted over a period of 3years show that the ULGCS trait was stable under field conditions and the foliage/floral organs of transgenic lines contained wild-type levels of gossypol and related terpenoids. Although it was a relatively small-scale study, we did not observe any negative effects on either the yield or quality of the fibre and seed in the transgenic lines compared with the nontransgenic parental plants. Compositional analysis was performed on the seeds obtained from plants grown in the field during 2009. As expected, the major difference between the ULGCS and wild-type cottonseeds was in terms of their gossypol levels. With the exception of oil content, the composition of ULGCS was similar to that of nontransgenic cottonseeds. Interestingly, the ULGCS had significantly higher (4%8%) oil content compared with the seeds from the nontransgenic parent. Field trial results confirmed the stability and specificity of the ULGCS trait suggesting that this RNAi-based product has the potential to be commercially viable. Thus, it may be possible to enhance and expand the nutritional utility of the annual cottonseed output to fulfil the ever-increasing needs of humanity.