Rapid Detection of the Mycobacterium tuberculosis Complex by Use of Quenching Probe PCR (geneCube)
Rapid Detection of the Mycobacterium tuberculosis Complex by Use of Quenching Probe PCR (geneCube)
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DOI：
10.1128/jcm.01654-12
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发表时间：
2012-11-01
影响因子：
9.4
通讯作者：
Iwano, Masayuki
中科院分区：
文献类型：
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作者：
Hida, Yukio;Hisada, Kyoko;Iwano, Masayuki
早期发现结核病对感染控制至关重要。geneCube（丰田）是一种新型的全自动基因分析仪，可以在60分钟内扩增目标dna。在这项研究中，我们评估了geneCube在临床标本中直接检测结核分枝杆菌复合体（MTBC）和鸟分枝杆菌复合体（MAC）的能力。然后将结果与常规培养、显微镜和Cobas扩增试验（罗氏）获得的结果进行比较。我们检查了来自69例培养阳性感染患者（73例，39例MTBC， 32例MAC和2例混合感染）和354例培养阴性患者（443例）的516份冷冻样本。使用geneCube检测MTBC的灵敏度为85.4%，特异性为99.8%，检测MAC的灵敏度为85.3%，特异性为99.8%。这些结果与使用Amplicor系统获得的结果相似，但获得的速度要快得多（使用geneCube的1小时比使用Amplicor系统的5.5小时）。因此，geneCube能够显著缩短分析时间，而不损失敏感性或特异性。
Early detection of tuberculosis (TB) is essential for infection control. The geneCube (Toyobo) is a novel fully automated gene analyzer that can amplify target DNAs within 60 min. In this study, we evaluated the ability of the geneCube to directly detect Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC) in clinical specimens. The results were then compared with those obtained using conventional culture, microscopy, and the Cobas Amplicor assay (Roche). We examined a total of 516 frozen samples from 69 patients who showed culture-positive infection (73 samples; 39 MTBC, 32 MAC, and 2 mixed infections) and from 354 patients who were culture negative (443 samples). Assays using the geneCube had a sensitivity of 85.4% and a specificity of 99.8% for detection of MTBC and a sensitivity of 85.3% and a specificity of 99.8% for detection of MAC. These results are similar to those obtained using the Amplicor system but were obtained much more rapidly (1 h with the geneCube versus 5.5 h with the Amplicor system). The geneCube thus enables a significant shortening of the assay time with no loss of sensitivity or specificity.