Development of a two-photon polymerization and optical tweezers microscope for fabrication and manipulation of microstructures
Development of a two-photon polymerization and optical tweezers microscope for fabrication and manipulation of microstructures
复制标题
开发用于微结构制造和操作的双光子聚合和光镊显微镜
DOI：
10.1117/12.875938
复制
发表时间：
2011
影响因子：
1.9
通讯作者：
S. Mohanty
中科院分区：
文献类型：
--
作者：
N. Ingle;S. Mohanty
我们报道了一种双光子聚合(TPP)显微镜的发展，用于微结构的微制造，它能够通过使用光钳进行光学操纵。该系统基于倒置尼康显微镜，其上后端耦合可调谐的钛宝石飞秒(FS)激光器。在模型锁定条件下，利用TPP在可光聚合合成材料中制备了纳米粒子和线材。通过聚焦的TPP激光光束的轴向定位，在衬底表面上方的期望高度上制作了一维结构(用作波导)。在锁模条件下，使用相同的可调谐激光微束作为光钳来固定纳米结构，即使在高粘性介质中也可以在固定之前对其进行操纵。TPP诱导结构的大小取决于飞秒激光强度和曝光量。此外，通过整形飞秒激光光束到线图案，可以在不扫描光束或平台的情况下制造线性1D结构，由于线钳在X和Y方向上的各向异性捕获力，其沿线强度分布保持对齐。使用双光子聚合光钳不仅可以实现聚合结构的原位校正定位，还可以将荧光微球(谐振器/检测器)与聚合波导集成在一起。
We report development of a two-photon polymerization (TPP) microscope, for micro-fabrication of microstructures, which is capable of optical manipulation by use of optical tweezers. The system is based on an inverted Nikon microscope with a tunable Ti: Sapphire femto-second (fs) laser coupled to the upper back port. While in modelocked condition, nanoparticles and wires were fabricated in photo-polymerizable synthetic materials using TPP. By axial positioning of the focused TPP laser beam, 1D-structures (for use as wave guide) were fabricated at desired height above the surface of the substrate. In the mode lock-OFF condition the same tunable laser microbeam was employed as optical tweezers to the hold the nanostructures and manipulate them even in highly viscous medium before immobilizing. Size of the TPP induced structure was found to depend on the fs laser intensity and exposure. Further, by shaping the fs laser beam to line pattern, linear 1D structures could be fabricated without scanning the beam or stage, which remain aligned along the line intensity profile due to anisotropic trapping force of the line tweezers in X and Y-directions. Use of optical tweezers with two-photon polymerization not only allowed in-situ corrective positioning of the polymerized structures, but also the integration of fluorescent microspheres (resonator/detector) with polymerized waveguide.