Acquired resistance to a nucleopolyhedrovirus in the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae) after selection by serial viral administration.
Acquired resistance to a nucleopolyhedrovirus in the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae) after selection by serial viral administration.
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通过连续病毒施用选择后，较小的茶托蛾 Adoxophyes honmai（鳞翅目：Tortricidae）获得了对核多角体病毒的抗性。
DOI：
10.1016/j.jip.2017.03.003
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Nakai;M.;Takahashi;K.;Iwata;K.;Tanaka;K.;Koyanagi;J.;Ookuma;A.;Takatsuka;J;Okuno;S.;Kunimi;Y.
本实验室用本氏剑水蚤核型多角体病毒（AdhoNPV）的LC 60或LC 70对本氏剑水蚤实验室群体进行了156代的抗性筛选。经3代选择，抗性品系（R系）与抗性品系（S1系和S2系）的LC_（50）值首次出现显著差异，随后抗性水平不断提高。根据R-和S1-菌株的LC_（50）值之比，获得性抗性的最高程度超过400，000倍。在第21代和第149代停止选择后，LC 50值没有显著下降，表明R株对AdhoNPV的抗性稳定。为了评估两种杆状病毒感染途径中哪一种受AdhoNPV抗性的影响，将AdhoNPV口服和血体腔内接种R-株的5龄幼虫，并将其敏感性与S-株的敏感性进行比较。接种5龄幼虫时，筛选株和分离株的LC_（25）值之比为91倍，而经口接种后LC_（25）值之比为107，000倍。这些结果表明，A. honmai破坏中肠原发感染和血腔继发感染。
A laboratory colony ofAdoxophyes honmaiwas selected for resistance over 156 generations by feeding neonate larvae of every generation with the LC60or LC70of its nucleopolyhedrovirus, Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV). A significant difference in LC50values between the selected (R-strain) and unselected (S1- and S2-strain) strains was first observed after three generations of selection, and the resistance level then increased continuously. The highest degree of acquired resistance, based on the ratio of the LC50values of R- and S1-strains, was more than 400,000-fold. After selection was stopped at either the 21st or the 149th generation, LC50values did not decrease significantly, suggesting that resistance of the R-strain to AdhoNPV was stable. To assess which of the two routes of baculovirus infection is affected by resistance to AdhoNPV, 5th instar larvae of the R-strain were inoculated orally and intrahemocoelically with AdhoNPV and their susceptibility was compared to that of S-strain. The ratio of the LC25values of selected and unselected strains was 91-fold when budded viruses were injected into 5th instar larvae, but was 107,000-fold after oral inoculation. These results indicate that the resistance mechanism of the R-strain ofA. honmaidisrupts both midgut primary infection and hemocoelic secondary infection.