Genome Editing in C. elegans and Other Nematode Species.
Genome Editing in C. elegans and Other Nematode Species.
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DOI：
10.3390/ijms17030295
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发表时间：
2016-02-26
影响因子：
5.6
通讯作者：
Sugi T
中科院分区：
文献类型：
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作者：
Sugi T
秀丽隐杆线虫是一种长1 mm的自由生活的线虫，是一种流行的模式动物，已被广泛用于各种生物过程的遗传研究。特征，使C。秀丽隐杆线虫是真核生物遗传学研究的一个强大的选择模型，包括其快速的生命周期（在20 °C下，从卵到成虫在3.5天内发育），注释良好的基因组，简单的形态（在雌雄同体中仅包含959个体细胞）和透明度（便于非侵入性荧光观察）。然而，早期在C.例如化学诱变和转座子的不精确切除，已经需要大规模的诱变筛选。为了避免这种费力和耗时的过程，基因组编辑技术已越来越多地用于线虫，包括C。briggsae和太平洋食心虫Pristionchus pacificus的遗传分析。在这里，我回顾了最近在线虫中使用锌指核酸酶（ZFN），转录激活因子样核酸酶（TALEN）和成簇规则间隔短回文重复序列（CRISPR）/Cas9的基因组编辑技术的进展，并提供了未来使用的前景。
Caenorhabditis elegans, a 1 mm long free-living nematode, is a popular model animal that has been widely utilized for genetic investigations of various biological processes. Characteristic features that make C. elegans a powerful model of choice for eukaryotic genetic studies include its rapid life cycle (development from egg to adult in 3.5 days at 20 °C), well-annotated genome, simple morphology (comprising only 959 somatic cells in the hermaphrodite), and transparency (which facilitates non-invasive fluorescence observations). However, early approaches to introducing mutations in the C. elegans genome, such as chemical mutagenesis and imprecise excision of transposons, have required large-scale mutagenesis screens. To avoid this laborious and time-consuming procedure, genome editing technologies have been increasingly used in nematodes including C. briggsae and Pristionchus pacificus, thereby facilitating their genetic analyses. Here, I review the recent progress in genome editing technologies using zinc-finger nucleases (ZFNs), transcriptional activator-like nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 in nematodes and offer perspectives on their use in the future.