In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair
In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair
复制标题
用于牙髓修复的纳米颗粒生物陶瓷糊剂的体外和体内评价
DOI：
10.1016/j.actbio.2014.08.014
复制
发表时间：
2014
影响因子：
9.7
通讯作者：
Peng Bin
中科院分区：
文献类型：
--
作者：
Zhu Lingxin;Yang Jingwen;Zhang Jie;Lei Dongqi;Xiao Lan;Cheng Xue;Lin Ying;Peng Bin
当活体牙髓受伤后暴露时，生物活性材料在促进牙髓修复方面发挥着重要作用。三氧化二矿物骨料是目前推荐用于纸浆修复程序的首选材料，尽管它有一些缺点，特别是处理不便。关于生物陶瓷介导的牙髓修复的早期事件和分子机制的信息还很少。我们的目的是表征和确定新型即用型纳米颗粒生物陶瓷 iRoot BP Plus 的磷灰石形成能力，并研究其对人牙髓干细胞 (DPSC) 体外募集的影响，以及其在牙髓修复体内模型中诱导牙本质桥形成的能力。研究发现iRoot BP Plus呈纳米尺寸，在体外具有优异的磷灰石形成能力。使用 iRoot BP Plus 提取物进行处理可促进 DPSC 的粘附、迁移和附着，并优化粘着斑形成（Vinculin、p-Paxillin 和 p-Focal 粘着激酶）和应力纤维组装。与体外结果一致，我们通过iRoot BP Plus观察到牙髓修复模型损伤部位均质牙本质桥的形成以及牙源性（牙本质唾液蛋白、牙本质基质蛋白1）和粘着斑分子（Vinculin、p-Paxillin）的表达。我们的研究结果为生物陶瓷介导的牙髓修复机制提供了宝贵的见解，而新型革命性的即用型纳米颗粒生物陶瓷糊剂在牙髓修复应用中显示出广阔的治疗潜力。
Bioactive materials play an important role in facilitating dental pulp repair when living dental pulp is exposed after injuries. Mineral trioxide aggregate is the currently recommended material of choice for pulp repair procedures though has several disadvantages, especially the inconvenience of handling. Little information is yet available about the early events and molecular mechanisms involved in bioceramic-mediated dental pulp repair. We aimed to characterize and determine the apatite-forming ability of the novel ready-to-use nanoparticulate bioceramic iRoot BP Plus, and investigate its effects on the in vitro recruitment of human dental pulp stem cells (DPSCs), as well as its capacity to induce dentin bridge formation in an in vivo model of pulp repair. It was found that iRoot BP Plus was nanosized and had excellent apatite-forming ability in vitro. Treatment with iRoot BP Plus extracts promoted the adhesion, migration and attachment of DPSCs, and optimized focal adhesion formation (Vinculin, p-Paxillin and p-Focal adhesion kinase) and stress fibre assembly. Consistent with the in vitro results, we observed the formation of a homogeneous dentin bridge and the expression of odontogenic (dentin sialoprotein, dentin matrix protein 1) and focal adhesion molecules (Vinculin, p-Paxillin) at the injury site of pulp repair model by iRoot BP Plus. Our findings provide valuable insights into the mechanism of bioceramic-mediated dental pulp repair, and the novel revolutionary ready-to-use nanoparticulate bioceramic paste shows promising therapeutic potential in dental pulp repair application.