A peroxisomal sterol carrier protein 2 (Scp2) contributes to lipid trafficking in differentiation and virulence of the insect pathogenic fungus Beauveria bassiana
A peroxisomal sterol carrier protein 2 (Scp2) contributes to lipid trafficking in differentiation and virulence of the insect pathogenic fungus Beauveria bassiana
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DOI：
10.1016/j.fgb.2021.103651
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发表时间：
2021-12-16
影响因子：
3
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
Lin, Hai-Yan;Pang, Meei-Yuan;Ying, Sheng-Hua
固醇载体蛋白2（SCP2）代表结合多种脂质的蛋白质家族，并且在细胞生理学中起重要作用。然而，它的生理作用在丝状真菌中很大程度上是未知的。在这项研究中，我们的功能特征的丝状昆虫病原真菌球孢白僵菌（BbScp2）的orthopathic Scp2基因。BbScp2是一种过氧化物酶体蛋白，对多种脂类具有不同的亲和力，对棕榈酸（PA）和麦角甾醇（ES）具有较强的亲和力。蛋白质与油酸（OA）和亚油酸（LA）之间没有明显的结合活性。BbScp2的消融并没有引起真菌生长的各种碳源的显着影响，但导致在孢子（49%）和芽生孢子产量（45%）的适度减少。此外，外源脂质可以恢复Delta BbScp 2突变株分生孢子中的缺陷物。BbScp2是所需的细胞膜功能的幼苗，其损失导致更显着的毒性下降，表明角质层感染试验比intrhemocoel注射试验。我们的研究结果表明，Scp 2连接的脂质运输的无性分化和毒性的B。巴西纳。
Sterol carrier protein 2 (SCP2) represents a family of proteins binding a variety of lipids and plays essential roles in cellular physiology. However, its physiological roles are largely unknown in filamentous fungi. In this study, we functionally characterized an orthologous Scp2 gene in the filamentous insect pathogenic fungus Beauveria bassiana (BbScp2). BbScp2 was verified to be a peroxisomal protein and displayed different affinities to various lipids, with strong affinity to palmitic acid (PA) and ergosterol (ES). No significant binding activity was detected between protein and oleic acid (OA) or linoleic acid (LA). Ablation of BbScp2 did not cause significant effects on fungal growth on various carbon sources, but resulted in a modest reduction in conidial (49%) and blastospore yield (45%). In addition, exogenous lipids could recover the defectives in conidiation of Delta BbScp2 mutant strain. BbScp2 was required for the cytomembrane functionality in germlings, and its loss resulted in a more significant decrease in virulence indicated by cuticle infection assay than intrahemocoel injection assay. Our findings indicate that Scp2 links the lipid trafficking to the asexual differentiation and virulence of B. bassiana.