IDENTIFICATION OF A MEMBRANE-ASSOCIATED INTERLEUKIN-1 IN MACROPHAGES
IDENTIFICATION OF A MEMBRANE-ASSOCIATED INTERLEUKIN-1 IN MACROPHAGES
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DOI：
10.1073/pnas.82.4.1204
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
UNANUE, ER
中科院分区：
文献类型：
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作者：
KURTJONES, EA;BELLER, DI;UNANUE, ER
在蛋白胨诱导的腹腔巨噬细胞上发现了具有强效胸腺细胞和T细胞刺激活性的表面膜相关白细胞介素1（IL-1）。在固定的巨噬细胞单层和未固定的巨噬细胞的分离膜上证明了IL-1活性。通过粘附和/或通过向巨噬细胞培养物中加入热灭活的单核细胞增生李斯特菌来诱导膜IL-1。巨噬细胞膜IL-1的功能和抗原性相似的可溶性IL-1，但它的表达可以暂时分离的IL-1分泌;膜IL-1诱导更早，持续时间比IL-1分泌在体外巨噬细胞培养。当培养的巨噬细胞已经停止分泌和膜表达的IL-1的再刺激加入热灭活李斯特菌，大量的膜IL-1诱导在可检测的IL-1分泌的情况下。膜IL-1似乎是一个完整的膜蛋白，因为它被洗涤剂溶解，但不被EDTA，高盐或低pH处理的膜洗脱。
A surface membrane-associated interleukin 1 (IL-1) with potent thymocyte and T cell stimulatory activity was found on peptone-elicited peritoneal macrophages. The IL-1 activity was demonstrated on both fixed macrophage monolayers and on isolated membranes from unfixed macrophages. Membrane IL-1 was induced by adherence and/or by adding heat-killed Listeria monocytogenes to macrophage cultures. The macrophage membrane IL-1 was similar functionally and antigenically to soluble IL-1, but its expression could be temporally dissociated from IL-1 secretion; membrane IL-1 was induced earlier and persisted longer than IL-1 secretion during in vitro macrophage culture. When cultured macrophages that had ceased both secretion and membrane expression of IL-1 were restimulated by adding heat-killed Listeria, substantial membrane IL-1 was induced in the absence of detectable IL-1 secretion. Membrane IL-1 appears to be an integral membrane protein since it was solubilized by detergent but was not eluted by EDTA, high salt or low pH treatment of the membranes.