A POTENTIAL POSITIVE FEEDBACK LOOP CONTROLLING CLN1 AND CLN2 GENE-EXPRESSION AT THE START OF THE YEAST-CELL CYCLE
A POTENTIAL POSITIVE FEEDBACK LOOP CONTROLLING CLN1 AND CLN2 GENE-EXPRESSION AT THE START OF THE YEAST-CELL CYCLE
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DOI：
10.1016/0092-8674(91)90394-e
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发表时间：
1991-05-31
期刊：
影响因子：
64.5
通讯作者：
TINKELENBERG, AH
中科院分区：
文献类型：
--
作者：
CROSS, FR;TINKELENBERG, AH
酿酒酵母的cln1、cln2和cln3基因组成了一个冗余的家族，对于从G1到S的相变是必不可少的。CLN1和CLN2mRNAs先前被证明受到交配信息素和细胞周期进程的负调控，而CLN3mRNAs则不受此调节。CLN3-2(DAF1-1)等位基因阻止细胞周期停滞和CLN1和CLN2mRNAs的关闭，以响应交配信息素，但仅在存在活性CDC28基因的情况下。用一个内部缺失的非功能cln2基因作为报告基因，证明了在没有交配信息素的情况下，cln2基因的有效表达既需要一个活性的CDC28基因，也需要至少一个功能基因。来自不起作用的cln1基因的mRNA也受到类似的调控。因此，CLN功能和CDC28活性共同刺激CLN1和CLN2mRNA水平，可能形成CLN1和CLN2mRNA表达的正反馈环。
The CLN1, CLN2, and CLN3 genes of S. cerevisiae form a redundant family essential for the G1-to-S phase transition. CLN1 and CLN2 mRNAs were previously shown to be negatively regulated by mating pheromone and by cell cycle progression out of G1, whereas CLN3 mRNA is not. The CLN3-2 (DAF1-1) allele prevents both cell cycle arrest and the turnoff of CLN1 and CLN2 mRNAs in response to mating pheromone, but only in the presence of an active CDC28 gene. An internally deleted nonfunctional cln2 gene was used as a reporter gene to demonstrate that in the absence of mating pheromone, efficient expression of cln2 mRNA requires both an active CDC28 gene and at least one functional CLN gene. mRNA from a nonfunctional cln1 gene was regulated similarly. Thus, CLN function and CDC28 activity jointly stimulate CLN1 and CLN2 mRNA levels, potentially forming a positive feedback loop for CLN1 and CLN2 expression.