Exon based amplified polymorphism (EBAP): a novel and universal molecular marker for plants
Exon based amplified polymorphism (EBAP): a novel and universal molecular marker for plants
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DOI：
10.1016/j.ejbt.2022.01.001
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发表时间：
2022-01
影响因子：
2.7
通讯作者：
Fa-qian Xiong;Jing Liu;Rong-hua Tang;Taiyi Yang;Xinghai Yang;Liang-qiong He;Zhuqiang Han;L. Qiu;Chenglin Zou;Xiumei Tang;C. Luo;Ruichun Zhong;Jing Jiang;Zhipeng Huang;Haining Wu;Junxian Liu;Xinhua He
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文献类型：
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作者：
Fa-qian Xiong;Jing Liu;Rong-hua Tang;Taiyi Yang;Xinghai Yang;Liang-qiong He;Zhuqiang Han;L. Qiu;Chenglin Zou;Xiumei Tang;C. Luo;Ruichun Zhong;Jing Jiang;Zhipeng Huang;Haining Wu;Junxian Liu;Xinhua He
背景外显子是基因的重要组成部分。然而，一直没有分子标记技术的基础上单引物扩增exonsregion.ResultsA新的分子标记技术称为基于外显子的扩增多态性（EBAP），使用单引物进行PCR。根据基因外显子区丰富的GC碱基设计单引物。单引物由填充序列、中间核心区序列和选择性碱基序列组成。填充序列共有6个碱基，可以是2A和2T，也可以是3A和3T。中间核心区序列由8个碱基组成，均为G和C，但至少需要2G或2C。三个选择性碱基中的第一个必须是A或T，第二个和第三个是任意碱基。由于引物设计的原则，这些单引物应该是通用的。将其应用于玉米、甘蔗、马铃薯、木薯、甘蓝、花生等作物的DNA多态性检测。结果表明，该方法在前5种作物中检测到了较丰富的DNA多态性，而在栽培花生中没有检测到。此外，用不同的单引物扩增花生cDNA，得到不同的带型。结论我们开发了一种新的和通用的分子标记技术。它简单、快速、高效。它不需要基因组序列信息。引用方式：Xiong F，Liu J，Tang R，et al. Exon based amplified polymorphism（EBAP）：A novel and universal molecular marker for plants. 56.第五十六章：一个人https://doi.org/10.1016/j.ejbt.2022.01.001.
BackgroundExons are an important part of genes. However, there has been no molecular marker technique based on single primer amplification developed for exons region.ResultsA novel molecular marker technique called exon based amplified polymorphism (EBAP) which used single primer to perform the PCR was developed. Single primers were designed based on the rich GC bases in the exon region of genes. The single primer consists of filling sequence, intermediate core region sequence and selective bases sequence. The filling sequence has a total of six bases, which can be 2A and 2T or 3A and 3T. The intermediate core region sequence consists of eight bases, all of which are G and C, but at least 2G or 2C are required. The first of the three selective bases must be either A or T, and the second and third are arbitrary bases. Due to the principle of primer design, these single primers should be universal. We applied it to the DNA polymorphisms detection of maize, sugarcane, potato, cassava, cabbage, and peanut. The results showed that it detected more abundant DNA polymorphisms in the first five crops, but not in the cultivated peanut. In addition, different band patterns were obtained by amplifying peanut cDNA with different single primers.ConclusionsWe have developed a novel and universal molecular marker technique. It is simple, fast and efficient. It does not require genomic sequence information. It can be widely used in germplasm identification, genetic diversity analysis, and molecular fingerprinting.How to cite: Xiong F, Liu J, Tang R, et al. Exon based amplified polymorphism (EBAP): A novel and universal molecular marker for plants. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2022.01.001.