Mass spectrometry of carbohydrates: newer aspects.
Mass spectrometry of carbohydrates: newer aspects.
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DOI：
10.1002/chin.200908280
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发表时间：
2009-02
影响因子：
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通讯作者：
J. Rodrigues;Adrian M. Taylor;D. Sumpton;J. C. Reynolds;R. Pickford;J. Thomas-Oates
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文献类型：
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作者：
J. Rodrigues;Adrian M. Taylor;D. Sumpton;J. C. Reynolds;R. Pickford;J. Thomas-Oates
出版商概要 本章讨论碳水化合物的质谱分析。现代质谱仪能够分析飞摩尔级别的物质，并能获得分子量远超100 kDa的复杂分子的高分子量。质谱仪可用于测定分子量以及生成碎片数据，以便在串联质谱实验中进行测序和结构解析。为了对来自生物基质的碳水化合物混合物进行全面分析，这些混合物呈现出高度的组成和结构异质性，需要一种多维方法。液相色谱或毛细管电泳（CE）与质谱（MS）的联用系统具有选择性高效分离的优势，同时还能从MS获得质量特异性、灵敏度和结构信息。碳水化合物CE分析所涉及的复杂性导致用于碳水化合物分析的在线CE - MS联用的实例非常少。研究发现，分离方法的三重联用能够鉴定在异质生物样品中发现的大量寡糖或糖型。
Publisher Summary This chapter discusses the mass spectrometry of carbohydrates. Modern mass spectrometers make it possible for substances in the femtomole range to be analyzed and for high molecular masses of complex molecules well over 100 kDa to be obtained. Mass spectrometers allow molecular mass determination and the generation of fragmentation data to allow sequencing and structure elucidation, generally in tandem mass-spectrometric experiments. To obtain a comprehensive analysis of carbohydrate mixtures from biological matrices that display a high degree of compositional and structural heterogeneity, a multidimensional approach is required. The hyphenated system of liquid chromatography or capillary electrophoresis (CE) with mass spectrometry (MS) provides the advantage of selective and efficient separation, with the mass specificity, sensitivity, and structural information gained from MS. The complexities involved in carbohydrate CE analysis have resulted in very few examples of on-line CE–MS coupling for carbohydrate analysis. It is found that triple hyphenation of separation methods can allow the identification of a large number of oligosaccharides or glycoforms found in heterogeneous biological samples.