Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats
Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats
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DOI：
10.1093/jn/137.8.1901
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发表时间：
2007-08-01
影响因子：
4.2
通讯作者：
Bueno, Lionel
中科院分区：
文献类型：
--
作者：
Eutamene, Helene;Lamine, Florence;Bueno, Lionel
应激事件导致肠道通透性和敏感性的改变。副干酪乳杆菌NCC2461 （Lpa）治疗可预防抗生素诱导的小鼠内脏痛觉过敏。本研究旨在评估3种益生菌菌株：乳酸双歧杆菌NCC362、约氏乳杆菌NCC533和Lpa对应激介导的结直肠痛觉过敏改变、肠道细胞旁通透性的影响，以及存在于乏培养基（SCM）中的细菌和/或细菌产物是否参与了有效菌株的抗伤性。在产后2-14天内，将幼鼠与母鼠分离3 h/d。在第13周，通过尿排泄Cr-51-EDTA的百分比来确定肠道细胞旁通透性，并通过腹肌肌电图评估内脏对结肠直肠膨胀（CRD）的敏感性。还分析了部分约束应激（PRS，限制身体运动2小时）下成年大鼠的内脏敏感性。大鼠分别在SCM或新鲜生长培养基中重悬益生菌2周作为对照。与对照组大鼠相比，母性剥夺显著增加了结肠对CRD的敏感性，并增强了肠道细胞旁通透性。只有Lpa治疗能显著改善应激性内脏疼痛，恢复正常的肠通透性。同样，在测试的3种益生菌中，只有Lpa可以预防prs介导的内脏痛觉过敏。Lpa SCM中存在的细菌和细菌产物对PRS的抗伤性都是必需的。该研究阐明了菌株特异性效应，并表明副干酪乳杆菌细菌和发酵和生长过程中产生的细菌产物之间存在协同相互作用，从而能够抑制prs诱导的大鼠超敏反应。
Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted Cr-51-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects arid suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation arid growth that confers the ability to suppress PRS-induced hypersensitivity in rats.