Isolation of an endophytic diazotroph, Klebsiella oxytoca, from sweet potato stems in Japan
Isolation of an endophytic diazotroph, Klebsiella oxytoca, from sweet potato stems in Japan
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DOI：
10.1080/00380768.2002.10408717
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发表时间：
2002-12-01
影响因子：
2
通讯作者：
Mochida, H
中科院分区：
文献类型：
--
作者：
Adachi, K;Nakatani, M;Mochida, H
在日本宫崎的宫城，田间生长的甘薯品种（Beniotome [BO]、Koganesengan [KS]和Shiroyutaka [SY]）的茎中，使用半固体无氮培养基，内生细菌的群体密度范围为10（2）至10（4）个细胞g（-1）鲜重样品。从茎中分离到11个菌株，其中BO-1和BO-5两个菌株在乙炔还原活性（ARA）试验中呈阳性反应。BO-1和BO-5是从黄瓜品种（cv.分别于1999年9月和2000年9月在Beniotome举行。这些分离物的形态生理学特征表明，BO-1和BO-5在马铃薯蔗糖琼脂斜面上显示出相似的菌落颜色，在改良的半固体培养基中产生气泡，酸化培养基，并且使用API 20 NE快速诊断试剂盒显示出相似的特征。BO-1菌株的16 SrRNA部分序列分析表明，BO-1菌株与产酸克雷伯氏菌（Klebsiellacoxytoca）的16 SrRNA同源性为100%（491 bp）。其余9株在ARA试验中呈阴性反应，轻微酸化或不酸化培养基。16 SrRNA部分序列分析表明，SY-2菌株与甲基杆菌属（Methylobacterium sp.）BO-8与血鞘氨醇单胞菌（Sphingomonassanguinis）的同源性为98.8%（419 bp）。
The population density of endophytic bacteria in the stem of field-grown sweet potato cultivars (Beniotome [BO], Koganesengan [KS], and Shiroyutaka [SY]) in Miyakonojo, Miyazaki, Japan, ranged from 10(2) to 10(4) cells g(-1) fresh weight sample using a semi-solid nitrogen-free medium. Eleven strains were isolated from the stems and two isolates, BO-1 and BO-5, showed a positive reaction in the acetylene reduction activity (ARA) test. BO-1 and BO-5 were isolated from cv. Beniotome in September 1999 and 2000, respectively. Morpho-physiological characterization of these isolates revealed that BO-1 and BO-5 showed a similar colony color in potato sucrose agar slants, produced bubbles in a modified semi-solid medium, acidified the medium, and displayed similar characteristics using the API 20NE rapid diagnostic kit. Partial sequence analysis of 16S rRNA from BO-1 revealed a 100% similarity (491 bp) to that of Klebsiella oxytoca. The other 9 isolates showed a negative reaction in the ARA test, slightly acidified or did not acidify the medium. Partial sequence analysis of the 16S rRNA revealed that the isolate SY-2 corresponded to Methylobacterium sp. (99.3% similarity for 1,241 bp), BO-3 to. Pantoea agglomerans (99.1% similarity for 469 bp), and BO-8 to Sphingomonas sanguinis (98.8% similarity for 419 bp).