Salidroside production by hairy roots of Rhodiola sachalinensis obtained after transformation with Agrobacterium rhizogenes
Salidroside production by hairy roots of Rhodiola sachalinensis obtained after transformation with Agrobacterium rhizogenes
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DOI：
10.1248/bpb.30.439
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发表时间：
2007-03-01
影响因子：
2
通讯作者：
Xu, Hongwei
中科院分区：
文献类型：
--
作者：
Zhou, Xiaofu;Wu, Yuxia;Xu, Hongwei
发根农杆菌诱导的毛状根生长快，被认为是遗传稳定的。这些毛状根可以作为一种有趣的材料来生产具有药用价值的次生代谢物。红景天苷是高山红景天根中的主要成分。在这里，我们提供了一个新的视角，为利用红景天根癌农杆菌诱导的毛状根生产红景天苷的前景和挑战增加了新的视角。为了提高红景天苷的产量，在LB型液体培养基中分别添加了前体(酪醇、酪氨酸和苯丙氨酸)和诱导子(黑曲霉、杂色云芝和灵芝)的最佳浓度。在MS液体培养基中添加激发子和利用化学补料产生的前体可以提高红景天苷的产量和生物量。诱导子和前体在液体培养中的最适浓度分别为0.05 mg/L和1 mmoL/L。
Hairy roots induced by Agrobacterium rhizogenes grow faster, and are considered as genetically stable. These hairy roots can be used as an interesting material for the production of secondary metabolites of pharmaceutical value. Salidroside has been identified as the major compounds from the roots of Rhodiola sachalinensis A. BOR. Here, we provide an update that adds new perspectives on the prospects and challenges of producing Salidroside from hairy roots induced by Agrobacterium rhizogene in Rhodiola sachalinensis A. BOR. For high salidroside production, the optimal concentration for precursor (Tyrosol, Tyrosine, and Phenylalanine) and elicitor (Aspergillus niger, Coriolus versicolor, and Ganoderma lucidum) was added in the LB liquid medium, respectively. The addition of elicitor in the liquid MS medium and the utilization of precursor from chemical feeding enhanced biomass accumulation and salidroside production. The optimal concentration for elicitor and precursor in the liquid medium was 0.05 mg/l and 1 mmol/l, respectively.