Biomechanical Properties of Mouse Carotid Arteries With Diet-Induced Metabolic Syndrome and Aging.
Biomechanical Properties of Mouse Carotid Arteries With Diet-Induced Metabolic Syndrome and Aging.
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DOI：
10.3389/fbioe.2022.862996
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发表时间：
2022
影响因子：
5.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Gkousioudi A;Yu X;Ferruzzi J;Qian J;Wainford RD;Seta F;Zhang Y
代谢综合征会增加心血管疾病的风险。随着年龄的增长，动脉逐渐萎缩;然而，它可能会因代谢综合征相关疾病的存在而恶化。在这项研究中，我们研究了饮食诱导的代谢综合征和衰老对小鼠颈总动脉（CCA）生物力学特性的联合影响。2月龄的雄性小鼠分别饲喂正常或高脂高糖（HFHS）饲料2个月（幼龄组）、8个月（成年组）和18-20个月（老龄组）。CCA被切除，并在体外进行双轴膨胀-伸展试验和柯西应力-拉伸关系，确定在圆周和纵向方向。采用四纤维族本构模型计算了CCAs的弹性储能，同时计算了材料在周向和纵向的刚度。我们的研究表明，衰老是影响成年和老年小鼠动脉重塑的主要因素，程度相似，无论饮食如何，僵硬表现为能量储存能力显著降低50%（p < 0.05），材料硬度和应力降低（p < 0.05）。另一方面，高脂高糖饮食通过影响环周材料刚度和应力（p < 0.05）导致糖尿病前期年轻组动脉重塑加速，最终被衰老进程所掩盖。这些发现对代谢综合征对年轻人群弹性动脉的影响具有重要意义。
Metabolic syndrome increases the risk of cardiovascular diseases. Arteries gradually stiffen with aging; however, it can be worsened by the presence of conditions associated with metabolic syndrome. In this study, we investigated the combined effects of diet-induced metabolic syndrome and aging on the biomechanical properties of mouse common carotid arteries (CCA). Male mice at 2 months of age were fed a normal or a high fat and high sucrose (HFHS) diet for 2 (young group), 8 (adult group) and 18–20 (old group) months. CCAs were excised and subjected to in vitro biaxial inflation-extension tests and the Cauchy stress-stretch relationships were determined in both the circumferential and longitudinal directions. The elastic energy storage of CCAs was obtained using a four-fiber family constitutive model, while the material stiffness in the circumferential and longitudinal directions was computed. Our study showed that aging is a dominant factor affecting arterial remodeling in the adult and old mice, to a similar extent, with stiffening manifested with a significantly reduced capability of energy storage by ∼50% (p < 0.05) and decreases in material stiffness and stress (p < 0.05), regardless of diet. On the other hand, high fat high sucrose diet resulted in an accelerated arterial remodeling in the young group at pre-diabetic stage by affecting the circumferential material stiffness and stress (p < 0.05), which was eventually overshadowed by aging progression. These findings have important implications on the effects of metabolic syndrome on elastic arteries in the younger populations.