Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease.
Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease.
复制标题
鉴定在牙周病患者中特异性表达的连翘坦纳氏菌抗原。
DOI：
10.1111/j.1574-6968.2007.00906.x
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发表时间：
2007
影响因子：
2.1
通讯作者：
Lee,Seok-Woo
中科院分区：
文献类型：
--
作者：
Yoo,JiYeon;Kim,HyeongChan;Zhu,Weidong;Kim,Seon-Mi;Sabet,Mojgan;Handfield,Martin;Hillman,Jeffrey;Progulske-Fox,Ann;Lee,Seok-Woo
由于有关其毒力因子的信息有限，连翘坦纳菌（一种推定的牙周病原体）的分子发病机制尚未得到充分阐明。在此，鉴定体内表达的T抗原。据报道，连翘是使用体内诱导抗原技术（IVIAT）进行的。在筛选的 13 000 个重组克隆中，鉴定出 16 个阳性克隆，它们与从牙周病患者获得的血清可重复反应。确定了其中 12 个体内诱导基因的 DNA 序列。 IVIAT 鉴定的 T 蛋白抗原。连翘包括：BspA，T 的明确毒力因子。连翘；参与管家功能的酶（tRNA 合成酶、甘氨酸羟甲基转移酶和葡萄糖苷葡萄糖水解酶）；参与组织破坏的酶（二肽基肽酶 IV）； DNA错配修复蛋白；和假定的功能未知的外膜蛋白。这些 IVIAT 鉴定的抗原的体内基因表达通过定量实时 PCR 分析得到证实。据作者所知，这是第一份使用 IVIAT inT 的报告。连翘。预计本研究中对 IVIAT 鉴定的体内诱导基因的详细分析将有助于更好地理解 T 介导牙周感染的分子机制。连翘。
Molecular pathogenesis ofTannerella forsythia, a putative periodontal pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here, identification ofin vivoexpressed antigens ofT. forsythiais reported usingin vivo-induced antigen technology (IVIAT). Among 13 000 recombinant clones screened, 16 positive clones were identified that reacted reproducibly with sera obtained from patients with periodontal disease. DNA sequences from 12 of thesein vivo-induced genes were determined. IVIAT-identified protein antigens ofT. forsythiainclude: BspA, a well-defined virulence factor ofT. forsythia; enzymes involved in housekeeping functions (tRNA synthetases, glycine hydroxymethyltransferase, and glucoside glucohydrolase); enzymes implicated in tissue destruction (dipeptidyl peptidase IV); a DNA mismatch repair protein; and putative outer membrane proteins of unknown function. Thein vivogene expression of these IVIAT-identified antigens was confirmed by a quantitative real-time PCR analysis. This is, to the best of the authors' knowledge, the first report using IVIAT inT. forsythia. It is anticipated that detailed analysis of thein vivo-induced genes identified by IVIAT in this study will lead to a better understanding of the molecular mechanisms mediating periodontal infection byT. forsythia.