Positively charged polymer polylysine-induced cell adhesion molecule redistribution in K562 cells
Positively charged polymer polylysine-induced cell adhesion molecule redistribution in K562 cells
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DOI：
10.1023/a:1008915305681
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发表时间：
1998-12-01
影响因子：
3.7
通讯作者：
Santini, MT
中科院分区：
文献类型：
--
作者：
Rainaldi, G;Calcabrini, A;Santini, MT
我们最近证明，如果通常在悬浮中生长的人K562红白血病细胞在聚赖氨酸包被的培养瓶中生长48小时，它们会粘附在这些瓶上，并以锚定依赖的方式生长。细胞膜电导率（离子在细胞膜上的传递）和膜介电常数（电荷在细胞膜上的静态分布）也发生了重要变化，这表明膜脂、蛋白质和多糖受到了扰动。为了更好地了解暴露于聚赖氨酸的K562细胞发生的变化，并且由于细胞粘附分子（CAMs）在细胞/细胞和细胞/基质相互作用以及细胞对周围环境的适应中发挥重要作用，研究了这些分子在暴露于聚赖氨酸后可能的重新分布。特别是CD54 （ICAM-1）、CD58 （LFA-3）和α (v) β(3)（玻璃体粘连蛋白受体）分子在不同生长时期分别利用流式细胞术和免疫荧光显微镜进行定量和定性研究。数据表明，在所有测试时间内，检测的cam没有显著的数量变化。此外，暴露48 h（和24 h）时均未观察到质变。然而，较短的治疗时间（30分钟，1和2小时）确实诱导了重要的CAM重组。结果似乎表明，这种CAM再分配循环可能部分地负责细胞对K562细胞新生长环境的适应以及所观察到的膜电学特性的变化。(C) 1998 Kluwer学术出版社。
We have recently demonstrated that if human K562 erythroleukemic cells, which normally grow in suspension, are grown in polylysine-coated culture flasks for 48 h, they adhere to these flasks and grow in an anchorage-dependent like manner. Important changes in both membrane conductivity (ionic transport across the cell membrane) and membrane permittivity (static distribution of charges across the cell membrane) were also observed, indicating perturbations in membrane lipids, proteins and polysaccharides. In order to better understand the changes occurring in K562 cells exposed to polylysine and because of the important role played by cell adhesion molecules (CAMs) in cell/cell and cell/substratum interactions, and in cellular adaptation to the surrounding environment, the possible redistribution of these molecules after exposure to polylysine were investigated. In particular, the CD54 (ICAM-1), CD58 (LFA-3) and alpha(v)beta(3) (vitronectin receptor) molecules were investigated at different times of growth both quantitatively and qualitatively utilizing flow cytometry and immunofluorescence microscopy, respectively. The data indicate that there were no significant quantitative variations in the CAMs examined at all the times tested. In addition, no qualitative changes were observed at 48 h (as well as 24 h) of exposure. However, shorter treatment times (30 min, 1 and 2 h) did induce important CAM reorganization. The results seem to demonstrate that this cycle of CAM redistribution may, in part, be responsible for cellular adaptation to the new growth environment of K562 cells and for the variations in membrane electrical properties observed. (C) 1998 Kluwer Academic Publishers.