Reduced expression of αGlcNAc in Barrett's oesophagus adjacent to Barrett's adenocarcinoma - a possible biomarker to predict the malignant potential of Barrett's oesophagus
Reduced expression of αGlcNAc in Barrett's oesophagus adjacent to Barrett's adenocarcinoma - a possible biomarker to predict the malignant potential of Barrett's oesophagus
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DOI：
10.1111/his.12296
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发表时间：
2014-03-01
期刊：
影响因子：
6.4
通讯作者：
Nakayama, Jun
中科院分区：
文献类型：
--
作者：
Iwaya, Yugo;Hasebe, Osamu;Nakayama, Jun
目的胃腺粘蛋白含有末端1，4-乙酰氨基葡萄糖残基(GlcNAc)的O-糖链。最近，我们发现胃腺粘蛋白中GlcNAc缺陷的小鼠自发发生胃腺癌，提示GlcNAc是一种胃癌的肿瘤抑制因子。然而，GlcNAc在Barrett‘s食道(BO)中的作用仍不清楚。方法与结果采用免疫组织化学方法检测35例BO邻近病变组织中GlcNAc、MUC6和CDX2的表达。同时分析了35例无BAC的食管鳞癌患者的BO病变作为对照。在与BAC相邻的BO中，GlcNAc相对于其支架MUC6的表达明显低于对照BO。BAC附近BO中GlcNAc表达的降低在肿瘤体积较小的患者中尤为显著(
AimsGastric gland mucin contains O-glycans exhibiting terminal 1,4-linked N-acetylglucosamine residues (GlcNAc). Recently we demonstrated that mice deficient in GlcNAc in gastric gland mucin develop gastric adenocarcinoma spontaneously, indicating that GlcNAc is a tumour suppressor for gastric cancer. However, the role of GlcNAc in Barrett's oesophagus (BO) remains unknown. In this study, we investigated whether reduced GlcNAc expression in BO is associated with development of Barrett's adenocarcinoma (BAC).Methods and resultsThirty-five BO lesions adjacent to BAC were examined by immunohistochemistry for GlcNAc, MUC6 and CDX2. As controls, 35 BO lesions without BAC obtained from patients with oesophageal squamous cell carcinoma were also analysed. Expression of GlcNAc relative to its scaffold MUC6 in BO adjacent to BAC was reduced significantly compared to control BO. Decreased GlcNAc expression in BO adjacent to BAC was particularly significant in patients with smaller tumour size (