Impaired salivary SIgA antibodies elicit oral dysbiosis and subsequent induction of alveolar bone loss
Impaired salivary SIgA antibodies elicit oral dysbiosis and subsequent induction of alveolar bone loss
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DOI：
10.1007/s00011-020-01418-x
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发表时间：
2020-11-09
影响因子：
6.7
通讯作者：
Kurita-Ochiai, Tomoko
中科院分区：
文献类型：
--
作者：
Chang, Emily;Kobayashi, Ryoki;Kurita-Ochiai, Tomoko
目的分泌型伊加（SIgA）通过中和毒素和病毒，抑制细菌对粘膜上皮细胞的粘附，在预防粘膜表面细菌和病毒感染中发挥重要作用。然而，唾液SIgA抗体（Abs）在调节口腔植物群中的作用仍不清楚。本研究的目的是评估口腔细菌，其代谢产物和牙周炎之间的关联IgA缺陷（伊加KO）和野生型（WT）的controlmice.Methods微计算机断层扫描（micro-CT）分析被用来评估牙槽骨吸收牙周炎的发展。使用下一代测序测定法确定唾液的细菌谱。结果与野生型相比，伊加KO小鼠唾液中链球菌的检出率明显降低，而聚集杆菌、放线杆菌和普雷沃菌的检出率明显升高。与相同年龄的WT对照小鼠相比，伊加KO小鼠的牙槽骨丢失水平显著增加，并且在牙槽骨表面发现破骨细胞浸润。代谢组学分析表明，伊加基因敲除小鼠的代谢产物在碳代谢、尿素循环和脂质代谢途径上的变异性大于野生型小鼠。结论唾液SIgA在调节和维持正常的口腔菌群，预防牙周病的发生中起重要作用。
Objective Secreted IgA (SIgA) plays a central role in preventing bacterial and viral infections on mucosal surfaces by neutralizing toxins and viruses and inhibiting bacterial attachment to epithelial cells. However, the role of salivary SIgA antibodies (Abs) in regulating oral flora is still unknown. This study aimed to evaluate the association among oral bacteria, their metabolites and periodontitis in IgA-deficient (IgA KO) and wild-type (WT) control mice.Methods Microcomputed tomography (micro-CT) analysis was used to assess alveolar bone resorption as a development of periodontitis. The bacterial profiles of saliva were determined using the next-generation sequencing assays. Furthermore, the metabolites in saliva were measured and compared using CE-TOFMS.Results Salivary microbiota of IgA KO mice revealed a remarkably decreased frequency of Streptococcus, and increased percentages of Aggregatibacer, Actinobacillus, and Prevotella at the genus level when compared with those of WT. Compared to WT control mice of the same age, the level of alveolar bone loss was significantly increased in IgA KO mice, and infiltration of osteoclasts was found on the surface of the alveolar bone. The metabolome profile indicated that the metabolites of IgA KO mice had greater variability in carbon metabolic, urea cycle, and lipid pathways than WT mice.Conclusion These results suggest that salivary SIgA plays an important role in regulating and maintaining normal oral microflora to prevent the development of periodontal disease.