An improved method to detect small amounts of radiation damage in DNA of eukaryotic cells.
An improved method to detect small amounts of radiation damage in DNA of eukaryotic cells.
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一种检测真核细胞 DNA 中少量辐射损伤的改进方法。
DOI：
10.1080/09553007914550961
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Weniger
中科院分区：
文献类型：
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作者：
P. Weniger
2.方法线性15- 30%蔗糖梯度（13 ml），其中含有1。将5 M NaCl、0.02 M Tris HCl（pH 8.0）、1 mM EDTANa 2和30µg/ml溴化乙锭与0.4 ml 1.5 M NaCl、0.1 M EDTA（pH 8.0）和0.7% Triton X-100重叠。将小鼠胸腺细胞（新鲜制备）悬浮至10 μ g/ml的浓度。
2. Method A linear 15-30 per cent sucrose gradient (13 ml) which contained 1. 5 M NaCl, 0.02 M Tris HCl pH 8.0, 1 mM EDTANa2 and 30µg/ml ethidium bromide was overlayered with 0.4 ml 1.5 M NaCl, 0.1 M EDTA pH 8.0 and 0.7 per cent Triton X-100. Mouse thymus cells (freshly prepared) were suspended to a concentration of 10'