Cardiovascular dysfunction in offspring of ovarian-hyperstimulated women and effects of estradiol and progesterone: a retrospective cohort study and proteomics analysis.
Cardiovascular dysfunction in offspring of ovarian-hyperstimulated women and effects of estradiol and progesterone: a retrospective cohort study and proteomics analysis.
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DOI：
10.1210/jc.2014-2349
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Gu-feng Xu;Jun-Yu Zhang;Hai-tao Pan;Shen Tian;Miao-E. Liu;Tian-tian Yu;Jingyi Li;Wei-Wen Ying;W. Yao;Xianhua Lin;Y. Lv;Wenzhi Su;Xiaohang Ye;Fang-hong Zhang;Jie-xue Pan;Ye Liu;Cheng-liang Zhou;Dan Zhang;Xin-Mei Liu;Yi-Min Zhu;J. Sheng;He-feng Huang
中科院分区：
文献类型：
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作者：
Gu-feng Xu;Jun-Yu Zhang;Hai-tao Pan;Shen Tian;Miao-E. Liu;Tian-tian Yu;Jingyi Li;Wei-Wen Ying;W. Yao;Xianhua Lin;Y. Lv;Wenzhi Su;Xiaohang Ye;Fang-hong Zhang;Jie-xue Pan;Ye Liu;Cheng-liang Zhou;Dan Zhang;Xin-Mei Liu;Yi-Min Zhu;J. Sheng;He-feng Huang
上下文
辅助生殖技术出生的儿童的心血管功能障碍一直是人们非常关注的问题。然而，卵巢过度刺激综合征(OHSS)是辅助生殖技术的一种并发症，其与心血管功能恶化的相关性和潜在机制仍不清楚。
目标
这项研究的目的是评估患有OHSS的母亲所生孩子的心血管功能，并调查潜在的调节因素(S)。
设计和设置
这是在一所大学医院招募的回溯性队列。
参与者和方法
应用多普勒超声对42例OHSS妇女所生子女、34例非OHSS母亲体外受精子女和48例自然受孕(SC)儿童(平均年龄∼4.5岁)的心血管功能进行了评价。在分娩时的孕周和出生体重方面，各组都是匹配的。对另一组来自OHSS和SC妊娠的脐动脉(两组均为3例)进行了用于相对和绝对定量标记蛋白质组学的等量标记。
结果
与非OHSS母亲和SC儿童相比，OHSS母亲的孩子在体外受精和SC儿童中二尖瓣峰值早期和晚期的比率显著降低，颈总动脉收缩和舒张期内径减小，血流介导的扩张受损。三组内膜-中层厚度和动脉僵硬指数相似。在蛋白质组学研究中，从OHSS和SC脐动脉中鉴定了1640个蛋白质，并选择了40个差异表达蛋白质进行进一步分析。雌二醇和孕酮被确定为激活的上游调节因子。
结论
卵巢过度刺激的妇女所生的孩子表现出心血管功能障碍。其潜在的机制可能涉及超生理水平的雌二醇和孕酮水平的影响。
CONTEXT
The cardiovascular dysfunction in children born with assisted reproductive technologies has been of great concern. However, the association of ovarian hyperstimulation syndrome (OHSS), a complication of assisted reproductive technologies, with worse cardiovascular functions and underlying mechanism remains unknown.
OBJECTIVES
The objective of the study was to assess the cardiovascular functions of children born to mothers with OHSS and investigate the underlying regulator(s).
DESIGN AND SETTING
This was a retrospective cohort recruited in a university hospital.
PARTICIPANTS AND METHODS
We assessed the cardiovascular functions by Doppler echography in 42 children born to OHSS women, 34 children of mothers with non-OHSS in vitro fertilization, and 48 spontaneously conceived (SC) children (mean age ∼ 4.5 y). Groups were matched for gestational age at delivery and birth weight. An isobaric tag for relative and absolute quantitation-labeled proteomics analysis was performed with another set of umbilical arteries from OHSS and SC pregnancies (n = 3 for both groups).
RESULTS
Children of OHSS mothers showed a significantly decreased mitral ratio of early to late mitral peak velocities, reduced systolic and diastolic diameters of common carotid arteries, and impaired flow-mediated dilation compared with non-OHSS in vitro fertilization and SC children. Intima-media thickness and arterial stiffness indices were similar in the three groups. In the proteomics study, 1640 proteins were identified from OHSS and SC umbilical arteries, and 40 differentially expressed proteins were selected for further analysis. Estradiol and progesterone were identified as activated upstream regulators.
CONCLUSIONS
Children born to ovarian-hyperstimulated women displayed cardiovascular dysfunctions. The underlying mechanisms may involve the effects of supraphysiological estradiol and progesterone levels.