Analysis of nucleotide-binding oligomerization domain proteins in a murine model of pneumococcal meningitis.
Analysis of nucleotide-binding oligomerization domain proteins in a murine model of pneumococcal meningitis.
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肺炎球菌脑膜炎小鼠模型中核苷酸结合寡聚结构域蛋白的分析。
DOI：
10.1186/s12879-014-0648-3
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发表时间：
2014-12-02
影响因子：
3.7
通讯作者：
Leng J
中科院分区：
文献类型：
--
作者：
Liu X;Han Q;Leng J
先天免疫系统通过其模式识别受体识别病原体。本研究的目的是调查核苷酸结合寡聚化结构域（NOD）蛋白，一个新的细菌模式识别受体家族，在宿主对革兰氏阳性细菌肺炎链球菌的反应中的作用。Sprague-Dawley大鼠通过脑池内注射活的S.对照组注射无菌生理盐水。感染后，进行实时PCR以确定编码NOD 1和NOD 2的mRNA的存在。在用活链球菌挑战感染后，还进行了NOD 1、NOD 2和NF-kB蛋白的定量分析。肺炎。采用酶联免疫吸附试验（ELISA）检测脑组织匀浆中TNF-α和IL-6水平。结果显示，S.肺炎性脑膜炎此外，感染活S.实验性肺炎链球菌脑膜炎中NOD 2介导的NF-κB活化也显著增加。同样，体内S.肺炎杆菌给药。这些结果表明，NOD 2参与了宿主对革兰氏阳性菌S. NOD 2可能在与肺炎球菌脑膜炎相关的CNS炎症的发生和/或进展中起重要作用。本文的在线版本（doi：10.1186/s12879-014-0648-3）包含补充材料，可供授权用户使用。
The innate immune system recognizes pathogens via its pattern recognition receptors. The objective of this study was to investigate the role of the nucleotide-binding oligomerization domain (NOD) proteins, a family of the novel bacterial pattern recognition receptors, in host responses to the gram-positive bacteria Streptococcus pneumoniae. Sprague–Dawley rats were infected via intracisternal injections of viable S. pneumoniae, and rats in the control group were injected with sterile saline. After infection, real-time PCR was performed to determine the presence of mRNAs encoding NOD1 and NOD2. Quantitative analyses of the NOD1, NOD2 and NF-kB proteins were also performed western blotting following challenge infections with viable S. pneumoniae. The TNF-α and IL-6 levels in brain homogenates were evaluated using enzyme-linked immunosorbent assays (ELISAs). The results revealed up-regulations of the mRNA and protein levels of NOD2 within the CNS of rats with S. pneumoniae meningitis. Moreover, the activation of NF-κB in the brain tissues following infection with live S. pneumoniae was also significantly increased, which indicates that NOD2 mediated NF-κB activation in experimental pneumococcal meningitis. Similarly, TNF-α and IL-6 levels were increased in the brain following in vivo S. pneumoniae administration. These results suggest that NOD2 is involved in the host response to the gram-positive bacteria S. pneumoniae in the CNS and that NOD2 might play an important role in the initiation and/or progression of CNS inflammation associated with pneumococcal meningitis. The online version of this article (doi:10.1186/s12879-014-0648-3) contains supplementary material, which is available to authorized users.
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影响因子：
4.8
作者：
Gutierrez, O;Pipaon, C;Fernandez-Luna, JL
通讯作者：
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