Iron-dependent vs. iron-independent cold-induced injury to cultured rat hepatocytes:: A comparative study in physiological media and organ preservation solutions
Iron-dependent vs. iron-independent cold-induced injury to cultured rat hepatocytes:: A comparative study in physiological media and organ preservation solutions
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DOI：
10.1016/j.cryobiol.2006.11.008
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发表时间：
2007-02-01
期刊：
影响因子：
2.7
通讯作者：
de Groot, Herbert
中科院分区：
文献类型：
--
作者：
Rauen, Ursula;Kerkweg, Uta;de Groot, Herbert
We previously described the entity of cold-induced apoptosis to rat hepatocytes and characterized its major, iron-dependent pathway. However, after cold incubation in some solutions, e.g. cell culture medium, hepatocytes show an additional, yet uncharacterized component of cold-induced injury. We here assessed the effects of organ preservation solutions on both components of cold-induced injury and tried to further characterize the iron-independent component. None of the preservation solutions (University of Wisconsin, histidine-tryptophan-ketoglutarate, Euro-Collins, histidine-lactobionate, sodium-lactobionate-sucrose and Celsior solutions) provided significant protection against cold-induced cell injury (LDH release after 24-h cold incubation/3 It rewarming > 65% for all solutions); three solutions even enhanced cold-induced injury. However, when the predominant iron-dependent mechanism was eliminated by the addition of iron chelators, all preservation solutions yielded hepatocyte protection that was clearly superior to the one obtainable in cell culture medium or Krebs-Henseleit buffer with iron chelators (LDH release after 24-h cold incubation/3 It rewarming
