Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells
Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells
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DOI：
10.1021/acs.analchem.5b01772
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发表时间：
2015-08-18
影响因子：
7.4
通讯作者：
Nagaoka, Tsutomu
中科院分区：
文献类型：
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作者：
Le, Dung Quynh;Morishita, Aya;Nagaoka, Tsutomu
我们开发了一种新的细菌检测技术，通过干燥沉积在电极上的细菌悬浮液。研究还发现，使用氧化铟锡（ITO）电极显著提高了伏安图的分辨率，使我们能够观察到两对氧化还原峰，每个峰对应于细菌细胞包膜中对类异戊二烯泛醌（UQn）和甲基萘醌（MKn）的吸附，在pH 7.0时，与aglagc11饱和的KCII相比，峰中电位分别为-0.015和-0.25 V。在革兰氏阴性菌和阳性菌中，大多数微生物都有明确的氧化还原峰，表明该方法可以在没有溶剂提取的情况下检测醌类。研究表明，该技术不仅可以用于细菌的检测，还可以用于分析在微生物中电子和质子转移中起重要作用的类异戊二烯醌。在这方面，目前的技术提供了一种比溶剂萃取更直接的方法，因为通过将含有目标细菌的悬浮液加热蒸发，将其应用于ITO电极上，可以在1分钟内制备一个样品。
We have developed a novel bacterial detection technique by desiccating a bacterial suspension deposited on an electrode. It was also found that the use of an indium-tin-oxide (ITO) electrode dramatically improved the resolution of the voltammogram, allowing us to observe two pairs of redox peaks, each assigned to the adsorption of isoprenoid ubiquinone (UQn) and menaquinone (MKn), which were present in the bacterial cell envelopes, giving midpeak potentials of -0.015 and -0.25 V versus AglAgC1lsaturated KCII at pH 7.0, respectively. Most of the microorganisms classified in both the Gram-negative and -positive bacteria gave well-defined redox peaks, demonstrating that this procedure made the detection of the quinones possible without solvent extraction. It has been demonstrated that the present technique can be used not only for the detection of bacteria, but also for profiling of the isoprenoid quinones, which play important roles in electron and proton transfer in microorganisms. In this respect, the present technique provides a much more straightforward way than the solvent extraction in that one sample can be prepared in 1 min by heat evaporation of a suspension containing the targeted bacteria, which has been applied on the ITO electrode.