Does acute behavioral testing reflect successful ischemia in rats with transient middle cerebral artery occlusion?
Does acute behavioral testing reflect successful ischemia in rats with transient middle cerebral artery occlusion?
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DOI：
10.1111/j.1747-4949.2011.00710.x
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发表时间：
2012-08-01
影响因子：
6.7
通讯作者：
Wiart, Marlene
中科院分区：
文献类型：
--
作者：
Chauveau, Fabien;Cho, Tae-Hee;Wiart, Marlene
大脑中动脉腔内闭塞模型呈现出梗死面积的内在变异性。在动脉闭塞期间经常进行行为评估以确认手术成功。目的和/或假设我们比较了行为测试和多模态磁共振成像在动脉闭塞期间识别成功手术动物的价值。方法采用线栓法建立大鼠大脑中动脉闭塞模型，在闭塞2 h内进行行为学评价（3种评分量表和粘合剂清除试验）和多模式磁共振成像（包括磁共振血管造影、弥散加权成像和灌注加权成像）。行为评估重复24？h后处死。结果急性表观弥散系数损伤体积与2，3，5-三苯基四氮唑氯化物梗死面积相关（r = 0.9999，P <0.01）。0.75，P = 0.001。0.02）和行为状态（r =？0.66，P = 0.001。0.05）第一天。相反，急性行为学检查和第一天的结果之间没有相关性（2，3，5-三苯基四氮唑氯化物梗死体积，r =？0.40，P = 0.001。行为学检查，r = 0.28; 0.39，P = 0.001。0.30）。第0天表观扩散系数体积（P？=？0.04），而行为学评估无显著性差异（P =？0.60），区分第一天皮质纹状体梗死的动物与皮质下梗死的动物。结论动脉闭塞期间进行的急性行为测试未能识别成功手术的动物。急性弥散磁共振成像可能更适合于评估和减少该模型中梗死面积的变异性。
Background Models of intraluminal middle cerebral artery occlusion present an intrinsic variability in infarct size. Behavioral evaluation is frequently performed during arterial occlusion to confirm success of surgery. Aims and/or hypothesis We compared the value of behavioral testing and multimodal magnetic resonance imaging performed during arterial occlusion for identifying successfully operated animals. Methods Rats were tested with behavioral assessment (using three scoring scales and the adhesive removal test) and multimodal magnetic resonance imaging (including magnetic resonance angiography, diffusion-weighted and perfusion-weighted imaging), both performed during the two-hours of middle cerebral artery occlusion using the intraluminal suture model. Behavioral assessment was repeated 24?h after reperfusion, followed by sacrifice. Results Acute apparent diffusion coefficient lesion volume was correlated with both 2,3,5-triphenyl tetrazolium chloride infarct size (r?=?0.75, P?=?0.02) and behavioral status (r?=?0.66, P?=?0.05) on day one. Conversely, no correlation was found between acute behavioral examination and day one outcomes (2,3,5-triphenyl tetrazolium chloride infarct volume, r?=?0.40, P?=?0.28; behavioral examination, r?=?0.39, P?=?0.30). Day zero apparent diffusion coefficient volumes (P?=?0.04), but not behavioral assessment (P?=?0.60), discriminated animals with day one corticostriatal infarcts from these with subcortical infarcts. Conclusions Acute behavioral testing performed during arterial occlusion fails to identify successfully operated animals. Acute diffusion magnetic resonance imaging may be more appropriate to assess and reduce infarct size variability in this model.