Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco
Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco
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将重建的酵母铁还原酶基因refre1引入烟草中
DOI：
10.1023/a:1004591012061
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发表时间：
1999
期刊：
影响因子：
4.9
通讯作者：
S. Mori
中科院分区：
文献类型：
--
作者：
Hiroyuki Oki;H. Yamaguchi;H. Nakanishi;S. Mori
Fe（III）在根表面的还原是策略I植物吸收Fe的强制性步骤。基因FRE1和FRE2负责酿酒酵母细胞膜中Fe获得的类似机制。将FRE1基因导入烟草（Nicotiana tabacum L.）CV. SR1）。然而，转基因烟草没有表现出额外的还原酶活性，因为来自该转基因烟草的FRE1转录物比预期的短。进一步的研究表明，FRE1基因的编码区是聚腺苷酸化的。然后，我们重建了FRE1基因的整个序列，并将其命名为refre 1（重建的FRE1）。将refre 1基因导入烟草植物中。转refre 1基因的转基因植物产生全长mRNA，并具有组成型铁还原酶活性。
Fe(III) reduction at the root surface is an obligatory step in Fe uptake used by Strategy-I plants. The genes FRE1 and FRE2 are responsible for a similar mechanism of Fe acquisition in the cell membrane of Saccharomyces cerevisiae. We introduced the FRE1 gene into tobacco plants (Nicotiana tabacum L. cv. SR1). However, the transgenic tobacco showed no additional reductase activity, because the FRE1 transcripts from this transgenic tobacco were shorter than expected. Further investigation revealed that the coding region of the FRE1 gene was polyadenylated. We then reconstructed the whole sequence of the FRE1 gene and named it refre1 (reconstructed FRE1). The refre1 gene was introduced into tobacco plants. The transgenic plants carrying the refre1 gene produced full length mRNA and had constitutive ferric reductase activity.