Cell size and the morphogenesis of wing hairs in Drosophila
Cell size and the morphogenesis of wing hairs in Drosophila
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DOI：
10.1002/1526-968x(200010)28:2
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发表时间：
2000-10
期刊：
影响因子：
1.5
通讯作者：
P. Adler;Jingchun Liu;J. Charlton
中科院分区：
文献类型：
--
作者：
P. Adler;Jingchun Liu;J. Charlton
果蝇翅膀上几乎所有的表皮细胞都只有一根表皮毛。这是在蛹中由微绒毛样细胞突起形成的，称为前毛。先前的实验表明，存在两种机制，确保只产生一根头发。一种是通过卷曲组织极性通路的作用，将原毛起始限制在细胞的一个小亚区。第二是建立一个确保主席完整性的制度。抑制肌动蛋白细胞骨架的突变和药物导致单个前毛分裂成多个更小的毛。我们报道，大的多倍体细胞产生多根毛发，因为它们形成多个独立的毛前起始中心，而且因为这些细胞产生的比正常头发大的毛发有分裂的倾向。我们发现，通过饥饿减少细胞大小可以部分抑制多倍体细胞的表型，而通过机械拉伸增加根尖细胞表面积也会导致多个毛前起始中心的形成。我们还表明，卷曲的组织极性途径在大多倍体细胞中是有功能的，即使它不能将毛前起始限制在细胞的一个小区域内。我们得出的结论是，这两种细胞系统在适应更大细胞尺寸的能力上都是有限的。创世纪28:82-91,2000。©2000 Wiley‐Liss, Inc。
Summary: Almost all epidermal cells on the Drosophila wing produce a single cuticular hair. This is formed in the pupae from a microvillus‐like cell projection called the prehair. Previous experiments have shown the existence of two mechanisms that ensure that only a single hair is made. One is the restriction of prehair initiation to a small subregion of the cell by the action of the frizzled tissue polarity pathway. The second is a system that ensures the integrity of the prehair. Mutations and drugs that inhibit the actin cytoskeleton lead to the splitting of a single prehair into multiple smaller hairs. We report that large polyploid cells produce multiple hairs both because they form multiple independent prehair initiation centers and because the larger than normal hairs these cells produce have a tendency to split. We show that reducing cell size by starvation partially suppresses the phenotype seen in polyploid cells and that increasing apical cell surface area by mechanical stretching also results in the formation of multiple prehair initiation centers. We also show that the frizzled tissue polarity pathway is functional in large polyploid cells even if it is unable to restrict prehair initiation to a small region of the cell. We conclude that both of these cellular systems are limited in their ability to scale to accommodate larger cell size. genesis 28:82–91, 2000. © 2000 Wiley‐Liss, Inc.