Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells
Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells
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DOI：
10.1016/j.biomaterials.2014.04.024
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发表时间：
2014-08-01
期刊：
影响因子：
14
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Liu, Li;Yoshioka, Momoko;Chen, Yong
纳米纤维明胶基质适合在无饲养层和无血清培养条件下长期扩增人类多能干细胞 (hPSC)。建立了具有不同处理参数集的组合文库，以评估 hPSC 在纳米纤维基质上的细胞粘附和生长速率方面的培养性能，并使用 Matrigel 作为对照。然后，将最佳条件应用于具有多个细胞系的 hPSC 的长期扩增，在 20 多次传代中显示出维持的多能性，而没有引入任何异常染色体。此外，这种方法使我们能够避免传代过程中的酶解和机械切割，从而促进更好的 hPSC 培养和长期扩增。 (C) 2014 Elsevier Ltd. 保留所有权利。
Nanofibrous gelatin substrates are suited for long-term expansion of human pluripotent stem cells (hPSCs) under feeder- and serum-free culture conditions. A combinatorial library with different sets of processing parameters was established to assess the culture performance of hPSCs on nanofibrous substrates in terms of cell adhesion and growth rate, using Matrigel as control. Then, the optimal conditions were applied to long-term expansion of hPSCs with several cell lines, showing a maintained pluripotency over more than 20 passages without introducing any abnormal chromosome. In addition, this approach allowed us to avoid enzymatic disassociation and mechanic cutting during passages, thereby promoting a better hPSC culture and long-term expansion. (C) 2014 Elsevier Ltd. All rights reserved.