Comprehensive Analysis of Protein Glycation Reveals Its Potential Impacts on Protein Degradation and Gene Expression in Human Cells.
Comprehensive Analysis of Protein Glycation Reveals Its Potential Impacts on Protein Degradation and Gene Expression in Human Cells.
复制标题
蛋白质糖化的综合分析揭示了其对人体细胞蛋白质降解和基因表达的潜在影响。
DOI：
10.1007/s13361-019-02197-4
复制
发表时间：
2019
影响因子：
3.2
通讯作者：
Wu,Ronghu
中科院分区：
文献类型：
--
作者：
Sun,Fangxu;Suttapitugsakul,Suttipong;Xiao,Haopeng;Wu,Ronghu
糖基化作为一种非酶蛋白质修饰，与衰老和慢性疾病，特别是糖尿病有关。对蛋白质糖基化的全面分析将有助于更好地理解其形成机制和生物学意义。在这项工作中，我们全面研究了人类细胞（HEK293T，Jurkat和MCF7细胞）中的蛋白质糖化。目前的研究结果表明，这种非酶促修饰不是随机的，细胞外区域和细胞核的蛋白质更频繁地被糖化。通过对糖基化蛋白质的系统性和位点特异性分析，我们可以研究蛋白质的一级序列和二级结构对糖基化的影响。此外，发现糖酵解途径中几乎所有酶都被糖基化，并提出了可能的机制。许多糖基化位点以前也被报道为乙酰化和泛素化位点，这强烈表明这种非酶修饰可能会干扰蛋白质降解和基因表达。目前的研究结果将有助于进一步研究蛋白质糖化在生物医学和临床研究。
Glycation as a type of non-enzymatic protein modification is related to aging and chronic diseases, especially diabetes. Global analysis of protein glycation will aid in a better understanding of its formation mechanism and biological significance. In this work, we comprehensively investigated protein glycation in human cells (HEK293T, Jurkat, and MCF7 cells). The current results indicated that this non-enzymatic modification was not random, and protein at the extracellular regions and the nucleus were more frequently glycated. Systematic and site-specific analysis of glycated proteins allowed us to study the effect of the primary sequences and secondary structures of proteins on glycation. Furthermore, nearly every enzyme in the glycolytic pathway was found to be glycated and a possible mechanism was proposed. Many glycation sites were also previously reported as acetylation and ubiquitination sites, which strongly suggested that this non-enzymatic modification may disturb protein degradation and gene expression. The current results will facilitate further studies of protein glycation in biomedical and clinical research.