Preparative methods for imaging plasmodesmata at super-resolution.
Preparative methods for imaging plasmodesmata at super-resolution.
复制标题
超分辨率成像胞间连丝的制备方法。
DOI：
10.1007/978-1-4939-1523-1_4
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Bell K
中科院分区：
文献类型：
--
作者：
Bell K
我们对胞间连丝的认识大多来自于对它们的视觉观察。光学显微镜是探索亚细胞结构的流行工具，但由于光的衍射特性，其分辨率有限。直径为50 nm的胞间连丝低于此限度，因此无法分辨。超分辨率显微镜的操作超出了传统光学显微镜的限制，提供了更详细的视图。虽然缺乏超微结构的分辨力的电子显微镜（EM），超分辨率显微镜有助于弥合传统的光学显微镜和EM之间的差距。在这里，我们提出了三个制备方法研究胞间连丝在超分辨率使用三维结构照明显微镜（3D-SIM）。
Much of our knowledge of plasmodesmata has come from the ability to visualize them. Light microscopy is a popular tool for exploring subcellular structures but is limited in its resolving power due to the diffractive properties of light. At 50 nm in diameter plasmodesmata are below this limit and so cannot be resolved. Super-resolution microscopy operates beyond the limits of conventional light microscopy affording a more detailed view. Although lacking the ultrastructural resolving power of the electron microscope (EM), super-resolution microscopy helps to bridge the gap between conventional light microscopy and EM.Here we present three preparative methods for studying plasmodesmata at super-resolution using 3D-structured illumination microscopy (3D-SIM).
影响因子：
56.9
作者：
Schermelleh, Lothar;Carlton, Peter M.;Sedat, John W.
通讯作者：
Sedat, John W.
DOI：
10.1046/j.1365-2818.1996.135417.x
发表时间：
1996-05-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Baskin, TI;Miller, DD;Hepler, PK
通讯作者：
Hepler, PK