Identification of a novel smooth muscle associated protein, smap2, upregulated during neointima formation in a rat carotid endarterectomy model
Identification of a novel smooth muscle associated protein, smap2, upregulated during neointima formation in a rat carotid endarterectomy model
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DOI：
10.1016/s0167-4781(02)00345-7
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发表时间：
2002-06-07
期刊：
影响因子：
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通讯作者：
Komurasaki, T
中科院分区：
文献类型：
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作者：
Nishimoto, S;Hamajima, Y;Komurasaki, T
主动脉平滑肌细胞的增殖是血管病变形成过程中的重要事件。为了寻找与新生内膜形成相关的新基因，我们构建了一个主动脉3‘端定向的cDNA文库。克隆了一个命名为Smap2(Smap2)的新基因。Smap2基因全长2914个碱基对，含有一个1338个碱基对的开放阅读框。斑点印迹分析表明，Smap2在大鼠主动脉中有特异性表达。SmAP2的推导氨基酸序列包含两个甲状腺球蛋白-1结构域、两个EF-Hand钙结合区和可能的信号肽。此外，我们还证明了在大鼠颈动脉内膜切除术模型中，Smap2基因在新生内膜形成过程中表达上调。这些发现提示Smap2可能参与了动脉粥样硬化的进展。(C)2002 Elsevier Science B.V.保留所有权利。
Proliferation of aortic smooth muscle cells is an important event in vascular lesion formation. To identify new genes that are involved in neointima formation, we constructed an aortic 3' -directed cDNA library. The novel cDNA of a gene designated smooth muscle associated protein 2 (smap2) was isolated. The full-length cDNA of smap2 is 2914 base pairs long and contains an open reading frame of 1338 base pairs. Dot blot analysis revealed that smap2 was expressed particularly in aorta. The deduced amino acid sequence of smap2 contains two thyroglobulin type-1 domains, two EF-hand calcium-binding domains and putative signal peptide. Furthermore, we demonstrated that smap2 mRNA was upregulated during neointima formation in a rat carotid endarterectomy model. These findings suggest that smap2 might be involved in the progression of atherosclerosis in aorta. (C) 2002 Elsevier Science B.V All rights reserved.