Plant catalase is imported into peroxisomes by pex5p but is distinct from typical PTS1 import
Plant catalase is imported into peroxisomes by pex5p but is distinct from typical PTS1 import
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DOI：
10.1093/pcp/pcn038
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发表时间：
2008-04-01
影响因子：
4.9
通讯作者：
Esaka, Muneharu
中科院分区：
文献类型：
--
作者：
Oshima, Yoshimi;Kamigaki, Akane;Esaka, Muneharu
我们之前已经证明，南瓜过氧化氢酶的靶向信号Cat1是内部PTS1（过氧化物酶体靶向信号1）样序列QKL，位于距C端13至11处，这与位于C端的典型PTS1 SKL基序不同。在这里，我们表明，Cat1 进入过氧化物酶体取决于拟南芥中的胞质 PTS 受体 Pex5p，类似于典型的 PTS1 输入，并且用于运输过氧化物酶体基质蛋白的其他成分，如 Pex14p、Pex13p、Pex12p 和 Pex10p 也有助于 Cat1 的输入。然而有趣的是，我们发现 Cat1 与 Pex5p 的 N 端结构域相互作用，但不与典型 PTS1 相互作用的 C 端结构域相互作用，这表明 Pex5p 以与典型 PTS1 不同的方式识别 Cat1。
We have previously demonstrated that the targeting signal of pumpkin catalase, Cat1, is an internal PTS1 (peroxisomal targeting signal 1)-like sequence, QKL, located at 13 to 11 from the C-terminus, which is different from the typical PTS1 SKL motif located in the C-terminus. Here we show that Cat1 import into peroxisome is dependent on the cytosolic PTS receptor, Pex5p, in Arabidopsis, similar to typical PTS1 import, and that other components for transport of peroxisomal matrix proteins such as Pex14p, Pex13p, Pex12p and Pex10p also contribute to the import of Cat1. Interestingly, however, we found that Cat1 interacts with the N-terminal domain of Pex5p, but not the C-terminal domain for interaction with the typical PTS1, revealing that Pex5p recognizes Cat1 in a manner distinct from typical PTS1.