Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer.
Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer.
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大鼠卵子在体外通过附睾精子受精以及移植后卵子的发育。
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
M. Chang
中科院分区：
文献类型：
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作者：
Y. Toyoda;M. Chang
摘要用PMSG和HCG处理的未成熟大鼠的新排卵卵在体外与从成熟雄性附睾尾回收的精子“授精”。使用改良的Krebs-Ringer碳酸氢盐溶液，补充葡萄糖、丙酮酸钠、乳酸钠、牛血清白蛋白和抗生素。卵和精子在0 μ m ~ 2. 4 ml介质，在矿物油下，在37 ° C下，在空气中的5%CO2的气相中。受精后5 ~ 5 h，精子开始穿透卵透明层，约2 h后精子头转化为雄性原核。受精后30 ~ 35小时，大多数穿透卵处于二细胞期。受精率为88%。7%（316/356），受精后30 ~ 46小时产卵率为75%。3%（174/231）。只有少数卵在进一步孵化后分裂到四细胞阶段。将203枚体外受精的二细胞卵移植到14只假孕大鼠的输卵管中。9名受体妊娠，获得43个胎儿和新生儿（占发育的21%）。
Summary. Newly ovulated eggs from immature rats treated with PMSG and HCG were `inseminated' in vitro with spermatozoa recovered from the cauda epididymidis of mature males. A modified Krebs-Ringer bicarbonate solution, supplemented with glucose, sodium pyruvate, sodium lactate, bovine serum albumin and antibiotics, was used. Eggs and spermatozoa were incubated in 0\m=.\4 ml medium under mineral oil at 37\s=deg\ C in a gas phase of 5% CO2 in air. Sperm penetration through the zona pellucida started 5 to 5\m=1/2\ hr after insemination and the transformation of sperm head(s) into the male pronucleus occurred about 2 hr later. Most of the penetrated eggs were at the two-cell stage 30 to 35 hr after insemination. The percentage of eggs undergoing fertilization was 88\m=.\7% (316/356) 7 to 20 hr after insemination and that offertilized eggs at 30 to 46 hr after insemination was 75\m=.\3% (174/231). Only a few eggs cleaved to the four-cell stage after further incubation. A total of 203 two-celled eggs fertilized in vitro were transferred to the oviducts of fourteen pseudopregnant rats on the first day of dioestrus. Nine recipients became pregnant and forty-three fetuses and newborn young were obtained (21% of development).