Experimental autoimmune encephalomyelitis is associated with changes of the microbiota composition in the gastrointestinal tract
Experimental autoimmune encephalomyelitis is associated with changes of the microbiota composition in the gastrointestinal tract
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DOI：
10.1038/s41598-020-72197-y
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发表时间：
2020-09-16
影响因子：
4.6
通讯作者：
Gaultier, Alban
中科院分区：
文献类型：
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作者：
Johanson, David M.;Goertz, Jennifer E.;Gaultier, Alban
已知肠道微生物组对免疫系统的变化敏感，特别是在多发性硬化症（MS）等自身免疫性疾病期间。我们的研究检查了在实验性自身免疫性脑脊髓炎（EAE）（MS的动物模型）期间发生的肠道微生物组的变化。我们在EAE进展的关键阶段收集了粪便样本，并用16 S V3-V4扩增子测序定量了微生物丰度。我们的数据分析表明，丰富的EAE期间减少，而属于梭菌科，瘤胃球菌科，消化链球菌科的其他EAE人群扩大。利用微生物共现网络进行的群落分析指出，这三个不断扩大的类群是肠道微生物组生态失调的潜在介质。我们还采用PICRUSt 2从原始微生物丰度数据中估算MetaCyc酶联合体（EC）途径丰度。从这一分析中，我们发现，一些负责生产免疫调节化合物的插补EC途径似乎在经历EAE的小鼠中富集。我们对结果的分析和解释提供了在EAE疾病进展过程中发生的肠道微生物组变化的详细情况，并有助于评估EAE作为MS患者肠道生态失调的可行模型。
The gut microbiome is known to be sensitive to changes in the immune system, especially during autoimmune diseases such as Multiple Sclerosis (MS). Our study examines the changes to the gut microbiome that occur during experimental autoimmune encephalomyelitis (EAE), an animal model for MS. We collected fecal samples at key stages of EAE progression and quantified microbial abundances with 16S V3-V4 amplicon sequencing. Our analysis of the data suggests that the abundance of commensal Lactobacillaceae decreases during EAE while other commensal populations belonging to the Clostridiaceae, Ruminococcaceae, and Peptostreptococcaceae families expand. Community analysis with microbial co-occurrence networks points to these three expanding taxa as potential mediators of gut microbiome dysbiosis. We also employed PICRUSt2 to impute MetaCyc Enzyme Consortium (EC) pathway abundances from the original microbial abundance data. From this analysis, we found that a number of imputed EC pathways responsible for the production of immunomodulatory compounds appear to be enriched in mice undergoing EAE. Our analysis and interpretation of results provides a detailed picture of the changes to the gut microbiome that are occurring throughout the course of EAE disease progression and helps to evaluate EAE as a viable model for gut dysbiosis in MS patients.