Rapid Screening of Gli2/3 Mutants Using the Flp-In System.
Rapid Screening of Gli2/3 Mutants Using the Flp-In System.
复制标题
使用 Flp-In 系统快速筛选 Gli2/3 突变体。
DOI：
10.1007/978-1-4939-2772-2_11
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Rohatgi,Rajat
中科院分区：
文献类型：
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作者：
Niewiadomski,Pawel;Rohatgi,Rajat
Gli2 and Gli3 respond to the Hedgehog (Hh) signal in mammals by undergoing posttranslational modifications and moving to the nucleus. The study of Gli proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of wild-type and mutant Gli proteins. This method is applicable to the study of effects of various mutations on Gli protein modifications and activity.
