Incorporation of ( 35 S) sulphate into brain constituents during development and the effects of thyroid hormone on myelination.
Incorporation of ( 35 S) sulphate into brain constituents during development and the effects of thyroid hormone on myelination.
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( 35 S) 硫酸盐在发育过程中掺入大脑成分以及甲状腺激素对髓鞘形成的影响。
DOI：
10.1016/0006-8993(71)90079-5
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发表时间：
1971
期刊：
影响因子：
2.9
通讯作者：
D. Wilson
中科院分区：
文献类型：
--
作者：
R. Balázs;B. Brooksbank;A. Patel;A. Johnson;D. Wilson
本文研究了三碘甲状腺原氨酸（T_3）对出生后9 ~ 26天大鼠脑内[~（35）S]硫酸盐沉积和脂质掺入的影响。脑苷脂沉积加速到一个小的程度，只有在17日龄之前。35 S掺入脑脂质在第17天达到最大值，但T3处理组的峰值不如对照组明显。在年龄的比放射性分离的[35 S]硫酸盐减少与T3的治疗，但减少不再明显时，数据表示的酸溶性35 S的浓度。也有一个异常，甚至在控制之间的速率的β-D-半乳糖苷的沉积和[35 S]硫酸盐的掺入到脂质期间，从17日至26日。结论是，[35 S]sulphatides的含量是不是一个有效的措施髓鞘沉积在发展过程中，除非掺入动力学of 35 S到brain也被考虑在内，调查还包括一个年龄分布of 35 S的馏分中含有粘多糖和酸溶性成分在对照组和T3处理的动物的大脑。含粘多糖组分中35 S的最大含量出现在第17天，而酸溶性组分中35 S的最大含量出现在第13天。结果表明，在评估35 S掺入相对速率时，必须考虑决定前体硫酸盐有效性的因素。
The effect of triiodothyronine (T3) on cerebroside deposition and on the incorporation of [35S]sulphate into lipids was studied in the rat brain between 9 and 26 days after birth. Cerebroside depositions was accelerated to a small extent and only before 17 days of age. The incorporation of35S into brain lipids was maximal at 17 days, but the peak was less pronounced in the T3-treated than in the control animals. At the age the specific radioactivity of the separated [35S]sulphatides was reduced by treatment with T3, but the reduction was no longer apparent when the data were expressed in terms of the concentration of acid-soluble35S. There was also an anomaly even in the control between the rates of cerebroside deposition and of the incorporation of [35S]sulphate into lipids during the period from 17 to 26 days. It was concluded that the content of [35S]sulphatides is not a valid measure of myelin deposition during development, unless the kinetics of incorporation of35S into the brain are also taken into account.The investigation also included a study of the age distribution of35S in the fractions containing mucopolysaccharides and acid-soluble constituents in the brains of both the control and T3-treated animals. The maximum content of35S occurred at 17 days in the fraction containing mucopolysaccharides but at 13 days in the acid-soluble fraction. The result showed that factors determining the availability of precursor sulphate must be considered in assessing relative rates of35S incorporation.