Development of transcriptomic biomarker signature in human saliva to detect lung cancer.
Development of transcriptomic biomarker signature in human saliva to detect lung cancer.
复制标题
开发人类唾液中的转录组生物标志物特征来检测肺癌。
DOI：
10.1007/s00018-012-1027-0
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发表时间：
2012-10
影响因子：
8
通讯作者：
Wong, David T. W.
中科院分区：
文献类型：
--
作者：
Zhang, Lei;Xiao, Hua;Zhou, Hui;Santiago, Silverio;Lee, Jay M.;Garon, Edward B.;Yang, Jieping;Brinkmann, Ole;Yan, Xinmin;Akin, David;Chia, David;Elashoff, David;Park, No-Hee;Wong, David T. W.
肺癌是全世界男性和女性癌症死亡的主要原因。由于大多数肺癌的症状是非特异性的，诊断大多是在晚期和进展阶段进行的，治疗效果较差。迫切需要有效的早期检测技术。新兴的唾液诊断领域可以提供科学可信、易于使用、非侵入性和具有成本效益的检测方法。最近的进展使我们能够开发出各种疾病从口腔系统性疾病的歧视性唾液生物标志物。在这项研究中，分析了肺癌患者的唾液转录组，并发现和预验证了七种高度区分的转录组唾液生物标志物（BRAF，CCNI，EGRF，FGF19，FRS2，GREB1和LZTS 1）。组合五种mRNA生物标志物（CCNI、EGFR、FGF19、FRS2和GREB1）的逻辑回归模型可以区分肺癌患者与正常对照受试者，在预验证样品集中产生0.925的AUC值，具有93.75%的灵敏度和82.81%的特异性。这些唾液mRNA生物标志物具有用于检测肺癌的区分能力。本报告提供了唾液生物标志物用于系统性疾病的非侵入性检测的概念证据。这些结果为唾液生物标志物在确定的临床背景下启动多中心验证奠定了基础。
Lung cancer is the leading cause of cancer death for both men and women worldwide. Since most of the symptoms found for lung cancer are nonspecific, diagnosis is mostly done at late and progressed stage with the consecutive poor therapy outcome. Effective early detection techniques are sorely needed. The emerging field of salivary diagnostics could provide scientifically credible, easy-to-use, non-invasive and cost-effective detection methods. Recent advances have allowed us to develop discriminatory salivary biomarkers for a variety of diseases from oral to systematic diseases. In this study, salivary transcriptomes of lung cancer patients were profiled and led to the discovery and pre-validation of seven highly discriminatory transcriptomic salivary biomarkers (BRAF, CCNI, EGRF, FGF19, FRS2, GREB1, and LZTS1). The logistic regression model combining five of the mRNA biomarkers (CCNI, EGFR, FGF19, FRS2, and GREB1) could differentiate lung cancer patients from normal control subjects, yielding AUC value of 0.925 with 93.75 % sensitivity and 82.81 % specificity in the pre-validation sample set. These salivary mRNA biomarkers possess the discriminatory power for the detection of lung cancer. This report provides the proof of concept of salivary biomarkers for the non-invasive detection of the systematic disease. These results poised the salivary biomarkers for the initiation of a multi-center validation in a definitive clinical context.
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影响因子：
3.7
作者：
Gao K;Zhou H;Zhang L;Lee JW;Zhou Q;Hu S;Wolinsky LE;Farrell J;Eibl G;Wong DT
通讯作者：
Wong DT
影响因子：
158.5
作者：
Kobayashi, S;Boggon, TJ;Halmos, B
通讯作者：
Halmos, B
影响因子：
11.2
作者：
Nonaka, D;Fabbri, A;Sozzi, G
通讯作者：
Sozzi, G
影响因子：
9.6
作者：
Gray, Robert D.;Duncan, Andrew;Boyd, A. Christopher
通讯作者：
Boyd, A. Christopher
影响因子：
4.4
作者：
Balko JM;Potti A;Saunders C;Stromberg A;Haura EB;Black EP
通讯作者：
Black EP